Evidence map›Paper›PMID 37333255›Full record

ArticlebioRxiv : the preprint server for biology2023

Anoctamin-1 is induced by TGF-beta and contributes to lung myofibroblast differentiation.

Eleanor B Reed, Shaina Orbeta, Bernadette A Miao, Albert Sitikov, Bohao Chen, Irena Levitan, Julian Solway, Gökhan M Mutlu, Yun Fang, Alexander A Mongin and 1 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Eleanor B ReedDepartment of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago, Chicago, IL, USA.
Shaina OrbetaDepartment of Neuroscience & Experimental Therapeutics, Albany Medical College, Albany, NY.
Bernadette A MiaoDepartment of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago, Chicago, IL, USA.
Albert SitikovDepartment of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago, Chicago, IL, USA.
Bohao ChenDepartment of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago, Chicago, IL, USA.
Irena LevitanDepartments of Medicine, Pharmacology and Bioengineering, University of Illinois at Chicago, Chicago, IL.
Julian SolwayDepartment of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago, Chicago, IL, USA.
Gökhan M MutluDepartment of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago, Chicago, IL, USA.
Yun FangDepartment of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago, Chicago, IL, USA.
Alexander A MonginDepartment of Neuroscience & Experimental Therapeutics, Albany Medical College, Albany, NY.ORCID 0000-0002-2501-3778
Nickolai O DulinDepartment of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago, Chicago, IL, USA.
University of Chicago · USAlbany Medical Center Hospital · USUniversity of Illinois Chicago · US

Funding

Fibroblast Biology and Pulmonary FibrosisR01HL149993 · NHLBI · UNIVERSITY OF CHICAGO · PI DULIN, NICKOLAI O · 2020 to 2023
$2.1M
Oxidative Stress and Pathological Glutamate Release in StrokeR01NS111943 · NINDS · ALBANY MEDICAL COLLEGE · PI MONGIN, ALEXANDER A · 2019 to 2023
$2.0M
NHLBI NIH HHS R01 HL149993NINDS NIH HHS R01 NS111943
6 · The paper itself

Abstract

Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by progressive scarring of the lungs and resulting in deterioration in lung function. Transforming growth factor-beta (TGF-β) is one of the most established drivers of fibrotic processes. TGF-β promotes transformation of tissue fibroblasts to myofibroblasts, a key finding in the pathogenesis of pulmonary fibrosis. We report here that TGF-β robustly upregulates the expression of the calcium-activated chloride channel Anoctamin-1 (ANO1) in human lung fibroblasts (HLF) at mRNA and protein levels. ANO1 is readily detected in fibrotic areas of IPF lungs in the same area with smooth muscle alpha-actin (SMA)-positive myofibroblasts. TGF-β-induced myofibroblast differentiation (determined by the expression of SMA, collagen-1 and fibronectin) is significantly inhibited by a specific ANO1 inhibitor, T16A

Indexed as

AKTAnoctamin-1RhoATGF-betawith-no-lysine (K) kinase

Identifiers

PMID37333255
PMCPMC10274757
OpenAlexW4379882325

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.