ArticleNPJ biofilms and microbiomes2023
Nitrate and a nitrate-reducing Rothia aeria strain as potential prebiotic or synbiotic treatments for periodontitis.
Article in NPJ biofilms and microbiomes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- The Influences of Oral Probiotics on the Immunometabolic Response During Pregnancy and Lactation: A Systematic Review.Nutrients · 2025Pooled it
- Evaluating the Role of Postbiotics in the Modulation of Human Oral Microbiota: A Randomized Controlled Clinical Trial.Probiotics and antimicrobial proteins · 2025Trial
- Microbiome and Inflammatory Biomarkers Associated With Palatal Wound Healing.Journal of periodontal research · 2025Trial
- Combined Effect of Nitrate Levels and Diabetes on the Risk of Severe Periodontitis: A Cross-sectional Study.Oral health & preventive dentistry · 2026Article
- Periodontitis and Endotoxemia: Clinical and Microbiological Insights.Journal of clinical periodontology · 2026Article
- Three novel Rothia species associated with Antarctic birds harbour novel biosynthetic gene clusters.FEMS microbiology ecology · 2026Article
- Integrated Host Genetics and Skin Microbiome Profiling Suggest an HLA-C-Peptostreptococcus Axis in Psoriasis.International journal of molecular sciences · 2026Article
- Dietary Nitrate Intake and 16S rRNA-Inferred Nitrite-Generating Capacity of the Subgingival Microbiome May Influence Glucose Metabolism: Results From the Oral Infections Glucose Intolerance and Insulin Resistance Study (ORIGINS).Journal of clinical periodontology · 2026Article
- Probiotics, Prebiotics, Synbiotics, Postbiotics, and Bioactive Agents in Modulating Harmful Oral Biofilms.Probiotics and antimicrobial proteins · 2026Review
- Combined effects of nitrate and antimicrobial compounds on in vitro subgingival biofilms.Scientific reports · 2026Article
- Epidemiological characteristics and co-occurrence patterns ofJournal of oral microbiology · 2026Article
- Unveiling potential driver taxa in subgingival plaque and their roles in mediating periodontitis progression.Journal of oral microbiology · 2026Article
- Metatranscriptomic insights into microbial network modulation and pathogen dynamics underlying healing outcomes in non-surgical periodontal treatment.ISME communications · 2026Article
- In-Depth Multi-Approach Analysis of WGS Metagenomics Data Reveals Signatures Potentially Explaining Features in Periodontitis Stage Severity.Dentistry journal · 2025Article
- Metabolic strategies that enable oral commensal persistence in a lower airway environment.mBio · 2025Article
- The butterfly effect: collateral damage and impacts of antimicrobial strategies on the oral microbiome.npj antimicrobials and resistance · 2025Review
- Oral and Gut Microbial Hubs Associated With Reaction Threshold Interact With Circulating Immune Factors in Peanut Allergy.Allergy · 2025Article
- MicroSTNet: a spatio-temporal graph-based framework for time-series microbiome analysis.Microbial genomics · 2025Article
- Characteristics of Subgingival Plaque Microbiome in Japanese Older Adults With Healthy Gingiva.Journal of clinical periodontology · 2025Article
- Microbiome data integration via shared dictionary learning.Nature communications · 2025Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A few studies indicate that nitrate can reduce dysbiosis from a periodontitis point of view. However, these experiments were performed on samples from healthy individuals, and it is unknown if nitrate will be effective in periodontal patients, where the presence of nitrate-reducing bacteria is clearly reduced. The aim of this study was to test the effect of nitrate and a nitrate-reducing R. aeria (Ra9) on subgingival biofilms of patients with periodontitis. For this, subgingival plaque was incubated with 5 mM nitrate for 7 h (n = 20) or 50 mM nitrate for 12 h (n = 10), achieving a ~50% of nitrate reduction in each case. Additionally, Ra9 was combined with 5 mM nitrate (n = 11), increasing the nitrate reduced and nitrite produced (both p < 0.05). The addition of nitrate to periodontitis communities decreased biofilm mass (50 mM > 5 mM, both p < 0.05). Five millimolar nitrate, 50 mM nitrate and 5 mM nitrate + Ra9 led to 3, 28 and 20 significant changes in species abundance, respectively, which were mostly decreases in periodontitis-associated species. These changes led to a respective 15%, 63% (both p < 0.05) and 6% (not significant) decrease in the dysbiosis index. Using a 10-species biofilm model, decreases in periodontitis-associated species in the presence of nitrate were confirmed by qPCR (all p < 0.05). In conclusion, nitrate metabolism can reduce dysbiosis and biofilm growth of periodontitis communities. Five millimolar nitrate (which can be found in saliva after vegetable intake) was sufficient, while increasing this concentration to 50 mM (which could be achieved by topical applications such as a periodontal gel) increased the positive effects. Ra9 increased the nitrate metabolism of periodontitis communities and should be tested in vivo.
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