Evidence map›Paper›PMID 37330445›Full record

ArticleProtoplasma2023

Characterization of cannabis strain-plant-derived extracellular vesicles as potential biomarkers.

Ayodeji O Ipinmoroti, Ja'kayla Turner, Elizabeth J Bellenger, Brennetta J Crenshaw, Junhuan Xu, Caitlin Reeves, Olufemi Ajayi, Ting Li, Qiana L Matthews

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protoplasma, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Ayodeji O IpinmorotiMicrobiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA.
Ja'kayla TurnerDepartment of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA.
Elizabeth J BellengerMicrobiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA.
Brennetta J CrenshawMicrobiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA.
Junhuan XuIndustrial Hemp & Specialty Crops Program, Alabama State University, Montgomery, AL, 36104, USA.
Caitlin ReevesIndustrial Hemp & Specialty Crops Program, Alabama State University, Montgomery, AL, 36104, USA.
Olufemi AjayiIndustrial Hemp & Specialty Crops Program, Alabama State University, Montgomery, AL, 36104, USA.
Ting LiDepartment of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA.
Qiana L MatthewsMicrobiology Program, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL, 36104, USA. qmatthews@alasu.edu.
Alabama State University · US

Funding

CDC HHS NU38OT000279
6 · The paper itself

Abstract

The scientific interest in cannabis plants' beneficial properties has recently sparked certain interest in the possible functional characterization of plant-derived extracellular vesicles (PDEVs). Establishing the most appropriate and efficient isolation procedure for PDEVs remains a challenge due to vast differences in the physio-structural characteristics of different plants within the same genera and species. In this study, we employed a crude but standard isolation procedure for the extraction of apoplastic wash fluid (AWF) which is known to contain the PDEVs. This method includes a detailed stepwise process of PDEV extraction from five (5) cultivars of cannabis plants, namely: Citrus (C), Henola (HA), Bialobrezenski (BZ), Southern-Sunset (SS), and Cat-Daddy (CAD). Approximately, 150 leaves were collected from each plant strain. In order to collect PDEV pellets, apoplastic wash fluid (AWF) was extracted from plants via negative pressure permeabilization and infiltration followed by high-speed differential ultracentrifugation. Particle tracking analysis of PDEVs revealed particle size distribution in the range of 20 to 200 nm from all plant strains, while PDEV total protein concentration from HA was higher than that of SS. Although HA-PDEVs' total protein was higher than SS-PDEVs, SS-PDEVs' RNA yield was higher than that of HA-PDEVs. Our result suggests that the cannabis plant strains contain EVs, and PDEV concentration from the cannabis plant could be age or strain dependent. Overall, the results provide a guide for the selection and optimization of PDEV isolation methods for future studies.

Indexed as

BiomarkersCannabisExtracellular VesiclesBiomarkersCannabidiolCannabisCultivarFiberPlant-derived extracellular vesicles

Identifiers

PMID37330445
PMCPMC10590282
OpenAlexW4381094800

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.