Evidence map›Paper›PMID 37330293›Full record

ArticleJournal of molecular biology2023

Structure of LARP7 Protein p65-telomerase RNA Complex in Telomerase Revealed by Cryo-EM and NMR.

Yaqiang Wang, Yao He, Yanjiao Wang, Yuan Yang, Mahavir Singh, Catherine D Eichhorn, Xinyi Cheng, Yi Xiao Jiang, Z Hong Zhou, Juli Feigon

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. A (Scientific) Lifetime Affair With Nucleic Acids.Journal of molecular biology · 2025
    Review
  4. Article
  5. A degenerate telomerase RNA directs telomeric DNA synthesis in lepidopteran insects.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Yaqiang WangDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095-1569, USA.
Yao HeDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095-1569, USA; Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095, USA.
Yanjiao WangDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095-1569, USA.
Yuan YangDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095-1569, USA.
Mahavir SinghDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095-1569, USA.
Catherine D EichhornDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095-1569, USA.
Xinyi ChengDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095-1569, USA.
Yi Xiao JiangDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095-1569, USA.
Z Hong ZhouDepartment of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095, USA; California NanoSystems Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Juli FeigonDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095-1569, USA. Electronic address: feigon@mbi.ucla.edu.
University of California, Los Angeles · USCalifornia NanoSystems Institute · US

Funding

Structural biology of telomerase and telomeresR35GM131901 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JULI FEIGON · 2019 to 2026
$5.7M
High-Resolution CryoEM Reconstruction of Large ComplexesR01GM071940 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI ZHOU, Z HONG · 2006 to 2025
$5.0M
Structural biology of 7SK RNP and its interaction with HIV-1 TatR01AI155170 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JULI FEIGON · 2020 to 2026
$4.8M
West/Midwest Consortium for High-Resolution Cryo Electron MicroscopyU24GM116792 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGBANDJE-MCKENNA, MAVIS, DOKLAND, TERJE · 2016 to 2020
$3.0M
HIGH-END CRYOEM INSTRUMENT FOR UCLA ELECTRON IMAGING CTR FOR NANOMACHINES: AIDSS10RR023057 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2006 to 2006
$1.6M
800 MHz NMR Console and Cryoprobe ReplacementS10OD025073 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI FEIGON, JULI · 2018 to 2018
$1.1M
Bruker Avance III Console and QCI Cryoprobe for a 600 MHz NMR SpectrometerS10OD016336 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CLUBB, ROBERT THOMPSON · 2014 to 2014
$600k
Direct Detection Device for atomic resolution cryoEM of macromolecular complexesS10OD018111 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2014 to 2014
$598k
NCRR NIH HHS S10 RR023057NIAID NIH HHS R01 AI155170NIGMS NIH HHS R01 GM071940NIGMS NIH HHS R35 GM131901NIGMS NIH HHS U24 GM116792NIH HHS S10 OD016336NIH HHS S10 OD018111NIH HHS S10 OD025073
6 · The paper itself

Abstract

La-related protein 7 (LARP7) are a family of RNA chaperones that protect the 3'-end of RNA and are components of specific ribonucleoprotein complexes (RNP). In Tetrahymena thermophila telomerase, LARP7 protein p65 together with telomerase reverse transcriptase (TERT) and telomerase RNA (TER) form the core RNP. p65 has four known domains-N-terminal domain (NTD), La motif (LaM), RNA recognition motif 1 (RRM1), and C-terminal xRRM2. To date, only the xRRM2 and LaM and their interactions with TER have been structurally characterized. Conformational dynamics leading to low resolution in cryo-EM density maps have limited our understanding of how full-length p65 specifically recognizes and remodels TER for telomerase assembly. Here, we combined focused classification of Tetrahymena telomerase cryo-EM maps with NMR spectroscopy to determine the structure of p65-TER. Three previously unknown helices are identified, one in the otherwise intrinsically disordered NTD that binds the La module, one that extends RRM1, and another preceding xRRM2, that stabilize p65-TER interactions. The extended La module (αN, LaM and RRM1) interacts with the four 3' terminal U nucleotides, while LaM and αN additionally interact with TER pseudoknot, and LaM with stem 1 and 5' end. Our results reveal the extensive p65-TER interactions that promote TER 3'-end protection, TER folding, and core RNP assembly and stabilization. The structure of full-length p65 with TER also sheds light on the biological roles of genuine La and LARP7 proteins as RNA chaperones and core RNP components.

Indexed as

Protozoan ProteinsTelomeraseTetrahymena thermophilaCryoelectron MicroscopyMagnetic Resonance SpectroscopyNucleic Acid ConformationRNARNA, ProtozoanProtozoan ProteinsRNARNA, ProtozoanTelomerasetelomerase RNALa moduleLa proteinpseudoknotRRMtelomerase

Identifiers

PMID37330293
PMCPMC10988774
OpenAlexW4380887656

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.