Evidence map›Paper›PMID 37329934›Full record

ArticleProtein expression and purification2023

Expression and purification of the mitochondrial transmembrane protein FAM210A in Escherichia coli.

Jared Hollinger, Jiangbin Wu, Kamel M Awayda, Mitchell R O'Connell, Peng Yao

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein expression and purification, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. FAM210A: An emerging regulator of mitochondrial homeostasis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jared HollingerAab Cardiovascular Research Institute, Department of Medicine, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA.
Jiangbin WuAab Cardiovascular Research Institute, Department of Medicine, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA.
Kamel M AwaydaDepartment of Biochemistry & Biophysics, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA.
Mitchell R O'ConnellDepartment of Biochemistry & Biophysics, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA; The Center for RNA Biology, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA.
Peng YaoAab Cardiovascular Research Institute, Department of Medicine, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA; Department of Biochemistry & Biophysics, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA; The Center for RNA Biology, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA; The Center for Biomedical Informatics, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA. Electronic address: peng_yao@urmc.rochester.edu.
University of Rochester · US

Funding

Programmable RNA-targeting CRISPR-Cas tools to study RNA biologyR35GM133462 · NIGMS · UNIVERSITY OF ROCHESTER · PI Mitchell O'Connell · 2019 to 2026
$3.6M
uORF-mediated Translational Control of Cardiac Transcription Factor ExpressionR01HL164584 · NHLBI · UNIVERSITY OF ROCHESTER · PI Peng Yao · 2023 to 2026
$2.0M
Deciphering the role of FAM210A in cardiac physiopathologyR01HL169432 · NHLBI · UNIVERSITY OF ROCHESTER · PI Peng Yao · 2023 to 2026
$1.9M
Role of FAK in vascular inflammationR01HL136432 · NHLBI · UNIVERSITY OF SOUTH ALABAMA · PI LIM, STEVE · 2017 to 2020
$1.6M
Role of miR-574-Fam210a axis in cardiac hypertrophy and remodelingR01HL132899 · NHLBI · UNIVERSITY OF ROCHESTER · PI YAO, PENG · 2018 to 2021
$1.5M
Translational Control of Cardiac FibrosisR01HL147954 · NHLBI · UNIVERSITY OF ROCHESTER · PI YAO, PENG · 2019 to 2022
$1.5M
NHLBI NIH HHS R01 HL132899NHLBI NIH HHS R01 HL136432NHLBI NIH HHS R01 HL147954NHLBI NIH HHS R01 HL164584NHLBI NIH HHS R01 HL169432NIGMS NIH HHS R35 GM133462
6 · The paper itself

Abstract

The protein Family with sequence similarity 210 member A (FAM210A) is a mitochondrial inner membrane protein that regulates the protein synthesis of mitochondrial DNA encoded genes. However, how it functions in this process is not well understood. Developing and optimizing a protein purification strategy will facilitate biochemical and structural studies of FAM210A. Here, we developed a method to purify human FAM210A with deleted mitochondrial targeting signal sequence using the MBP-His

Indexed as

Escherichia coliPeptide Elongation Factor TuHEK293 CellsHumansMembrane ProteinsMitochondrial ProteinsRecombinant ProteinsMembrane ProteinsMitochondrial ProteinsPeptide Elongation Factor TuRecombinant ProteinsFAM210AMitochondriaProtein synthesisTranslationTransmembrane

Identifiers

PMID37329934
PMCPMC11143474
OpenAlexW4380758732

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.