ArticleScience advances2023
Super-resolution vibrational imaging based on photoswitchable Raman probe.
Article in Science advances, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- AI-Assisted Coherent Raman Scattering Microscopy for Clinical Translation.Chemical & biomedical imaging · 2026Review
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Article
- 4Pi stimulated Raman scattering for label-free super-resolution chemical imaging.Science advances · 2026Article
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Functional Raman Probes for Detecting Enzyme Activities Based on Aggregation Control.Analytical chemistry · 2025Article
- 4-Pi Stimulated Raman Scattering for Label-free Super-resolution Chemical Imaging.bioRxiv : the preprint server for biology · 2025Article
- Quantum theory of stimulated Raman scattering microscopy.Chemical physics reviews · 2025Review
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Label-free nanoscopy of cell metabolism by ultrasensitive reweighted visible stimulated Raman scattering.Nature methods · 2025Article
- Wide-field bond-selective fluorescence imaging: from single-molecule to cellular imaging beyond video rate.Optica · 2025Article
- Uses of coherent Raman scattering microscopy in neuroscience.Frontiers in neuroscience · 2025Review
- Advances in Super-resolution Stimulated Raman Scattering Microscopy.Chemical & biomedical imaging · 2024Review
- Super-resolved coherent anti-Stokes Raman scattering microscopy by coherent image scanning.Nature communications · 2024Article
- Surpassing the Diffraction Limit in Label-Free Optical Microscopy.ACS photonics · 2024Review
- Click-free imaging of carbohydrate trafficking in live cells using an azido photothermal probe.Science advances · 2024Article
- Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control.Nature communications · 2024Article
Corrections and comments
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Authors and funding
18 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Super-resolution vibrational microscopy is promising to increase the degree of multiplexing of nanometer-scale biological imaging because of the narrower spectral linewidth of molecular vibration compared to fluorescence. However, current techniques of super-resolution vibrational microscopy suffer from various limitations including the need for cell fixation, high power loading, or complicated detection schemes. Here, we present reversible saturable optical Raman transitions (RESORT) microscopy, which overcomes these limitations by using photoswitchable stimulated Raman scattering (SRS). We first describe a bright photoswitchable Raman probe (DAE620) and validate its signal activation and depletion characteristics when exposed to low-power (microwatt level) continuous-wave laser light. By harnessing the SRS signal depletion of DAE620 through a donut-shaped beam, we demonstrate super-resolution vibrational imaging of mammalian cells with excellent chemical specificity and spatial resolution beyond the optical diffraction limit. Our results indicate RESORT microscopy to be an effective tool with high potential for multiplexed super-resolution imaging of live cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.