ArticlePhenomics (Cham, Switzerland)2023
Sample Collection, DNA Extraction, and Library Construction Protocols of the Human Microbiome Studies in the International Human Phenome Project.
Article in Phenomics (Cham, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A gut microbiome-lipid axis in early pregnancy is associated with metabolic dysregulation and diabetes risk.iMeta · 2026Article
- The Role of Gut Microbiota in the Association between Air Pollution and Cognitive Function in Older Adults.Environmental health perspectives · 2026Article
- Healthful plant-based diet, gut enterotype, and cognition in a rural Chinese elderly cohort: A longitudinal multi-omics study.Cell reports. Medicine · 2026Article
- Multi-kingdom profiling reveals altered gut phage-bacteria-metabolite interactions in MASLD.Nature communications · 2026Article
- Basic Microbiome Analysis: Analytical Steps from Sampling to Sequencing.Microorganisms · 2026Review
- Association between new dietary index for gut microbiota and lung cancer: A population-based study.Medicine · 2025Article
- Segatella copri and gut microbial ammonia metabolism contribute to chronic kidney disease pathogenesis.Nature microbiology · 2025Article
- Nasal microbiome in relation to olfactory dysfunction and cognitive decline in older adults.Translational psychiatry · 2025Article
- Impact of DNA Extraction Methods on Gut Microbiome Profiles: A Comparative Metagenomic Study.Phenomics (Cham, Switzerland) · 2025Article
- Culture dependent and independent approaches reveal the role of specific bacteria in human skin aging.iMetaOmics · 2024Article
- Enhancing colorectal cancer screening in high-risk population through fecal immunochemical test surveillance: Results from a surveillance program.Cancer medicine · 2024Article
- Gut microbial features and circulating metabolomic signatures of frailty in older adults.Nature aging · 2024Article
- Cancer and the Microbiome of the Human Body.Nutrients · 2024Review
- Comparison of the full-length sequence and sub-regions of 16S rRNA gene for skin microbiome profiling.mSystems · 2024Article
- Ketogenic diet-induced bile acids protect against obesity through reduced calorie absorption.Nature metabolism · 2024Article
- Article
- A piezoelectric-driven microneedle platform for skin disease therapy.Innovation (Cambridge (Mass.)) · 2024Article
- Low-Frequency Ultrasound Sensitive Piezo1 Channels Regulate Keloid-Related Characteristics of Fibroblasts.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Association of cigarette smoking with oral bacterial microbiota and cardiometabolic health in Chinese adults.BMC microbiology · 2023Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human microbiome plays a crucial role in human health. In the past decade, advances in high-throughput sequencing technologies and analytical software have significantly improved our knowledge of the human microbiome. However, most studies concerning the human microbiome did not provide repeatable protocols to guide the sample collection, handling, and processing procedures, which impedes obtaining valid and timely microbial taxonomic and functional results. This protocol provides detailed operation methods of human microbial sample collection, DNA extraction, and library construction for both the amplicon sequencing-based measurements of the microbial samples from the human nasal cavity, oral cavity, and skin, as well as the shotgun metagenomic sequencing-based measurements of the human stool samples among adult participants. This study intends to develop practical procedure standards to improve the reproducibility of microbiota profiling of human samples. Supplementary Information: The online version contains supplementary material available at 10.1007/s43657-023-00097-y.
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