Evidence map›Paper›PMID 37324585›Full record

ArticleFrontiers in molecular neuroscience2023

Identification of miRNAs regulating MAPT expression and their analysis in plasma of patients with dementia.

Paola Piscopo, Margherita Grasso, Valeria Manzini, Andrea Zeni, Michele Castelluzzo, Francesca Fontana, Giuseppina Talarico, Anna Elisa Castellano, Roberto Rivabene, Alessio Crestini and 3 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in molecular neuroscience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Paola PiscopoDepartment of Neuroscience, Istituto Superiore di Sanità, Rome, Italy.
Margherita GrassoDepartment of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.
Valeria ManziniDepartment of Neuroscience, Istituto Superiore di Sanità, Rome, Italy.
Andrea ZeniDepartment of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.
Michele CastelluzzoDepartment of Physics, University of Trento, Trento, Italy.
Francesca FontanaDepartment of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.
Giuseppina TalaricoDepartment of Human Neuroscience, University of Rome "Sapienza", Rome, Italy.
Anna Elisa CastellanoDepartment of Neurology, IRCCS Neuromed Institute, Pozzilli (IS), Italy.
Roberto RivabeneDepartment of Neuroscience, Istituto Superiore di Sanità, Rome, Italy.
Alessio CrestiniDepartment of Neuroscience, Istituto Superiore di Sanità, Rome, Italy.
Giuseppe BrunoDepartment of Human Neuroscience, University of Rome "Sapienza", Rome, Italy.
Leonardo RicciDepartment of Physics, University of Trento, Trento, Italy.
Michela A DentiDepartment of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.
University of Trento · ITIstituto Superiore di Sanità · ITSapienza University of Rome · ITIstituto Neurologico Mediterraneo · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Dementia is one of the most common diseases in elderly people and hundreds of thousand new cases per year of Alzheimer's disease (AD) are estimated. While the recent decade has seen significant advances in the development of novel biomarkers to identify dementias at their early stage, a great effort has been recently made to identify biomarkers able to improve differential diagnosis. However, only few potential candidates, mainly detectable in cerebrospinal fluid (CSF), have been described so far. Methods: We searched for miRNAs regulating MAPT translation. We employed a capture technology able to find the miRNAs directly bound to the MAPT transcript in cell lines. Afterwards, we evaluated the levels of these miRNAs in plasma samples from FTD ( Results: Firstly, we found all miRNAs that interact with the MAPT transcript. Ten miRNAs have been selected to verify their effect on Tau levels increasing or reducing miRNA levels by using cell transfections with plasmids expressing the miRNAs genes or LNA antagomiRs. Following the results obtained, miR-92a-3p, miR-320a and miR-320b were selected to analyse their levels in plasma samples of patients with FTD and AD respect to HCs. The analysis showed that the miR-92a-1-3p was under-expressed in both AD and FTD compared to HCs. Moreover, miR-320a was upregulated in FTD vs. AD patients, particularly in men when we stratified by sex. Respect to HC, the only difference is showed in men with AD who have reduced levels of this miRNA. Instead, miR-320b is up-regulated in both dementias, but only patients with FTD maintain this trend in both genders. Conclusions: Our results seem to identify miR-92a-3p and miR-320a as possible good biomarkers to discriminate AD from HC, while miR-320b to discriminate FTD from HC, particularly in males. Combining three miRNAs improves the accuracy only in females, particularly for differential diagnosis (FTD vs. AD) and to distinguish FTD from HC.

Indexed as

Alzheimer’s diseasebiomarkersdementiaFrontotemporal dementiaMAPTmicroRNAmicroRNA-capture affinity technology

Identifiers

PMID37324585
PMCPMC10266489
OpenAlexW4378902387

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.