Evidence map›Paper›PMID 37323577›Full record

ArticleCell reports methods2023

Engineering CpG island DNA methylation in pluripotent cells through synthetic CpG-free ssDNA insertion.

Joshua Tompkins, Elizabeth Lizhar, Alireza Shokrani, Xiwei Wu, Jordan Berley, Diba Kamali, Deborah Hussey, Jonas Cerneckis, Tae Hyuk Kang, Jinhui Wang and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 1 institution in 1 country.

Joshua TompkinsDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
Elizabeth LizharDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
Alireza ShokraniDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
Xiwei WuIntegrative Genomics Core, City of Hope, Duarte, CA 91010, USA.
Jordan BerleyDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
Diba KamaliDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
Deborah HusseyDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
Jonas CerneckisDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
Tae Hyuk KangIntegrative Genomics Core, City of Hope, Duarte, CA 91010, USA.
Jinhui WangIntegrative Genomics Core, City of Hope, Duarte, CA 91010, USA.
Walter TsarkDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
Defu ZengDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
Swetha GodathaDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
Rama NatarajanDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
Arthur RiggsDepartment of Diabetes Complications and Metabolism, Diabetes and Metabolism Research Institute, City of Hope, Duarte, CA 91010, USA.
City of Hope · US

Funding

Transcriptional Regulation by Angiotensin II in Vascular Smooth Muscle CellsR01HL106089 · NHLBI · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI NATARAJAN, RAMA · 2011 to 2024
$6.6M
NHLBI NIH HHS R01 HL106089
6 · The paper itself

Abstract

Cellular differentiation requires global changes to DNA methylation (DNAme), where it functions to regulate transcription factor, chromatin remodeling activity, and genome interpretation. Here, we describe a simple DNAme engineering approach in pluripotent stem cells (PSCs) that stably extends DNAme across target CpG islands (CGIs). Integration of synthetic CpG-free single-stranded DNA (ssDNA) induces a target CpG island methylation response (CIMR) in multiple PSC lines, Nt2d1 embryonal carcinoma cells, and mouse PSCs but not in highly methylated CpG island hypermethylator phenotype (CIMP)+ cancer lines.

Indexed as

DNA MethylationNeoplasmsAnimalsCpG IslandsDNA, Single-StrandedEpithelial CellsMiceDNA, Single-StrandedcardiomyocyteCas9CG islandDNA methylationepigenetic editingESCMLH1P53pluripotent stem cellsthymic epithelial cell

Identifiers

PMID37323577
PMCPMC10261899
OpenAlexW4380870802

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.