ArticlePlant biotechnology journal2023
CRISPR/Cas9-mediated simultaneous mutation of three salicylic acid 5-hydroxylase (OsS5H) genes confers broad-spectrum disease resistance in rice.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Molecular Bases and Genetic Design of Rice Disease Resistance for Optimized Yield and Sustainable Agriculture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Climate Warming and Plant Disease: Mechanistic Insights Into Pathogenic Stress Resilience.Plant, cell & environment · 2026Review
- CRISPR/Cas9-Mediated Editing ofPlants (Basel, Switzerland) · 2026Article
- Negative Regulators of Rice Agronomic Traits: Functional Insights and Applications in Genome Editing-Based Breeding.Plant biotechnology journal · 2026Review
- Advances in Genome Editing for Plant Disease Resistance Breeding.Plants (Basel, Switzerland) · 2026Review
- Genome-Wide Identification of Pineapple AcINH Genes and Functional Characterization ofPlants (Basel, Switzerland) · 2026Article
- Multiplex Gene Editing and Effect Analysis of Yield, Fragrance, and Blast Resistance Genes in Rice.Genes · 2026Article
- Hormone priming and metabolic engineering of phytohormone crosstalk in rice under combined biotic and abiotic stresses: a multi-omics perspective for climate-resilient crop development.Frontiers in plant science · 2026Review
- Linking the interaction of Salicylates and Jasmonates for stress resilience in plants.Stress biology · 2025Review
- Biotechnological breakthroughs in rice disease management: an overview from transgenics to CRISPR.Molecular biology reports · 2025Review
- Molecular Insights into Rice Immunity: Unveiling Mechanisms and Innovative Approaches to Combat Major Pathogens.Plants (Basel, Switzerland) · 2025Review
- The rational design of a Rhodamine fluorescent probe enables the selective detection and bioimaging of salicylic acid in plants under abiotic stress.Plant biotechnology journal · 2025Article
- UGT74B5-mediated glucosylation atHorticulture research · 2025Article
- The Role of Genetic Resistance in Rice Disease Management.International journal of molecular sciences · 2025Review
- CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops.Frontiers in genome editing · 2025Review
- Recent advances in innovative strategies for plant disease resistance breeding.Frontiers in plant science · 2025Review
- Molecular breeding approaches for sustainable rice blast management: recent advances and challenges.Frontiers in plant science · 2025Review
- Exploring the molecular mechanisms of rice blast resistance and advances in breeding for disease tolerance.Molecular biology reports · 2024Review
- The molecular dynamics between reactive oxygen species (ROS), reactive nitrogen species (RNS) and phytohormones in plant's response to biotic stress.Plant cell reports · 2024Review
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
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15 authors.
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Abstract
Salicylic acid (SA) is an essential plant hormone that plays critical roles in basal defence and amplification of local immune responses and establishes resistance against various pathogens. However, the comprehensive knowledge of the salicylic acid 5-hydroxylase (S5H) in rice-pathogen interaction is still elusive. Here, we reported that three OsS5H homologues displayed salicylic acid 5-hydroxylase activity, converting SA into 2,5-dihydroxybenzoic acid (2,5-DHBA). OsS5H1, OsS5H2, and OsS5H3 were preferentially expressed in rice leaves at heading stage and responded quickly to exogenous SA treatment. We found that bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) strongly induced the expression of OsS5H1, OsS5H2, and OsS5H3. Rice plants overexpressing OsS5H1, OsS5H2, and OsS5H3 showed significantly decreased SA contents and increased 2,5-DHBA levels, and were more susceptible to bacterial blight and rice blast. A simple single guide RNA (sgRNA) was designed to create oss5h1oss5h2oss5h3 triple mutants through CRISPR/Cas9-mediated gene mutagenesis. The oss5h1oss5h2oss5h3 exhibited stronger resistance to Xoo than single oss5h mutants. And oss5h1oss5h2oss5h3 plants displayed enhanced rice blast resistance. The conferred pathogen resistance in oss5h1oss5h2oss5h3 was attributed to the significantly upregulation of OsWRKY45 and pathogenesis-related (PR) genes. Besides, flg22-induced reactive oxygen species (ROS) burst was enhanced in oss5h1oss5h2oss5h3. Collectively, our study provides a fast and effective approach to generate rice varieties with broad-spectrum disease resistance through OsS5H gene editing.
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