ArticleThe ISME journal2023
Bacteriophage-mediated lysis supports robust growth of amino acid auxotrophs.
Article in The ISME journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
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- Limits to the evolution of metabolic dependency in spatially structured microbial communities.iScience · 2026Article
- Bacteriophage-mediated cell lysis externalizes a metabolically valuable nutrient to broadly modulate bacterial communities.The ISME journal · 2026Article
- Spatial structure: shaping the ecology and evolution of microbial communities.FEMS microbiology reviews · 2026Review
- Genomic safety, bacteriocinogenic potential, and probiotic-associated traits ofFrontiers in microbiology · 2026Article
- Widely-distributed freshwater microorganisms with streamlined genomes co-occur in cohorts with high abundance.Scientific reports · 2025Article
- The Corrinoid Model for Dissecting Microbial Community Interactions Across Scales.Annual review of microbiology · 2025Review
- Metabolic remodeling of microorganisms by mobile genetic elements alters mutualistic community composition.mSystems · 2025Article
- Analysis of (p)ppGpp metabolism and signaling using a dynamic luminescent reporter.PLoS genetics · 2025Article
- Metabolic ecology of microbiomes: Nutrient competition, host benefits, and community engineering.Cell host & microbe · 2025Review
- Skin-associatedmSphere · 2025Article
- Vitamin auxotrophies shape microbial community assembly on model marine particles.The ISME journal · 2025Article
- Vitamin BISME communications · 2025Article
- Phage-induced disturbance of a marine sponge microbiome.Environmental microbiome · 2024Article
- Viral communities in a pH>10 serpentinite-like environment: insight into diversity and potential roles in modulating the microbiomes by bioactive vitamin BApplied and environmental microbiology · 2024Article
- Soil microbial community response to corrinoids is shaped by a natural reservoir of vitamin BbioRxiv : the preprint server for biology · 2024Article
- Skin-associatedbioRxiv : the preprint server for biology · 2024Article
- Ecological dependencies and the illusion of cooperation in microbial communities.Microbiology (Reading, England) · 2024Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Microbial communities host many auxotrophs-organisms unable to synthesize one or more metabolites required for their growth. Auxotrophy is thought to confer an evolutionary advantage, yet auxotrophs must rely on other organisms that produce the metabolites they require. The mechanisms of metabolite provisioning by "producers" remain unknown. In particular, it is unclear how metabolites such as amino acids and cofactors, which are found inside the cell, are released by producers to become available to auxotrophs. Here, we explore metabolite secretion and cell lysis as two distinct possible mechanisms that result in the release of intracellular metabolites from producer cells. We measured the extent to which secretion or lysis of Escherichia coli and Bacteroides thetaiotaomicron amino acid producers can support the growth of engineered Escherichia coli amino acid auxotrophs. We found that cell-free supernatants and mechanically lysed cells provide minimal levels of amino acids to auxotrophs. In contrast, bacteriophage lysates of the same producer bacteria can support as many as 47 auxotroph cells per lysed producer cell. Each phage lysate released distinct levels of different amino acids, suggesting that in a microbial community the collective lysis of many different hosts by multiple phages could contribute to the availability of an array of intracellular metabolites for use by auxotrophs. Based on these results, we speculate that viral lysis could be a dominant mechanism of provisioning of intracellular metabolites that shapes microbial community structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.