ArticleNature plants2023
Whole-mount smFISH allows combining RNA and protein quantification at cellular and subcellular resolution.
Article in Nature plants, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Unlocking the full potential of spatial omics in plants: practical challenges, solutions, and a path forward.The Plant cell · 2026Review
- Transcriptional activity during seed imbibition is conserved, robust and required for germination.Nature communications · 2026Article
- Integrating single-cell omic techniques to resolve the spatio-temporal complexity of arbuscular mycorrhizal symbiosis.Journal of experimental botany · 2026Review
- Quantitative RNA spatial profiling using single-molecule RNA FISH on plant tissue cryosections.Plant communications · 2026Article
- Why "Where" Matters as Much as "How Much": Single-Cell and Spatial Transcriptomics in Plants.International journal of molecular sciences · 2025Review
- The ZAT14 family promotes cell death and regulates expansins to affect xylem formation and salt tolerance in Arabidopsis.The Plant cell · 2025Article
- Long Noncoding RNAs as Emerging Regulators of Seed Development, Germination, and Senescence.International journal of molecular sciences · 2025Review
- Translational Regulation of Plant Stress Responses: Mechanisms, Pathways, and Applications in Bioengineering.Annual review of phytopathology · 2025Review
- Deterministic patterns in single-cell transcriptomic data.NPJ systems biology and applications · 2025Article
- Engineering Plant Cell Fates and Functions for Agriculture and Industry.ACS synthetic biology · 2024Review
- RASP: Optimal Single Puncta Detection in Complex Cellular Backgrounds.The journal of physical chemistry. B · 2024Article
- A proxitome-RNA-capture approach reveals that processing bodies repress coregulated hub genes.The Plant cell · 2024Article
- Review
- Reference genes for quantitative Arabidopsis single molecule RNA fluorescence in situ hybridization.Journal of experimental botany · 2023Article
- Imaging of Molecular and Developmental Responses to Abiotic Stresses in Reproductive Tissues.Physiologia plantarumReview
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5 authors at 1 institution in 1 country.
Funding
Abstract
Multicellular organisms result from complex developmental processes largely orchestrated through the quantitative spatiotemporal regulation of gene expression. Yet, obtaining absolute counts of messenger RNAs at a three-dimensional resolution remains challenging, especially in plants, owing to high levels of tissue autofluorescence that prevent the detection of diffraction-limited fluorescent spots. In situ hybridization methods based on amplification cycles have recently emerged, but they are laborious and often lead to quantification biases. In this article, we present a simple method based on single-molecule RNA fluorescence in situ hybridization to visualize and count the number of mRNA molecules in several intact plant tissues. In addition, with the use of fluorescent protein reporters, our method also enables simultaneous detection of mRNA and protein quantity, as well as subcellular distribution, in single cells. With this method, research in plants can now fully explore the benefits of the quantitative analysis of transcription and protein levels at cellular and subcellular resolution in plant tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.