ArticleAnalytical and bioanalytical chemistry2023
Sensitive and discriminative detection of cysteine by a Nile red-based NIR fluorescence probe.
Article in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 12 citations in OpenAlex.
- A Coumarin-Based Probe for Sequential ON-OFF-ON Detection of CuBiosensors · 2026Article
- TAPP-based fluorescent probes for selective cysteine detection: insights into structure-reactivity relationships.RSC advances · 2026Article
- Construction of a mitochondria-targeted probe to monitor cysteine levels in cancer cells and zebrafish.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024Article
- Sensing Mechanism of Cysteine Specific Fluorescence Probes and Their Application of Cysteine Recognition.Chemical & biomedical imaging · 2024Review
- Poly-β-cyclodextrin strengthen PrAnalytical and bioanalytical chemistry · 2024Article
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Authors and funding
11 authors at 7 institutions in 2 countries.
Funding
Abstract
Cysteine (Cys) is a significant biological mercaptan that achieves key roles in several important physiological processes, such as reversible redox homeostasis in living organisms. Abnormal levels of Cys in the human body are directly related to many diseases. In this work, we constructed a sensitive sensor (Cys-NR) by connecting a Cys recognition group to a Nile red derivative. Due to photo-induced electron transfer (PET), the Cys-NR probe showed little fluorescence at 650 nm. With the addition of Cys to the assay solution, the chlorine unit of the probe was substituted by the thiol group of Cys. Further, the amino and sulfhydryl groups in cysteine underwent an intramolecular rearrangement, which led to the Cys-NR probe water solution turning from colorless to pink with an enhancement in fluorescence. The red fluorescence at 650 nm increased about 20 times. Based on the turn-on signal, a selective Cys detection method is developed. The probe signal is not affected by various potential interferences or other competing biothiols and the limit of detection (LOD) is determined to be 0.44 μM. In addition, the probe is further employed for imaging of Cys in live cells, revealing good biological imaging ability that could provide a new way of intracellular Cys detection.
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