Evidence map›Paper›PMID 37317792›Full record

ArticleStem cells (Dayton, Ohio)2023

ZIC1 Dictates Osteogenesis Versus Adipogenesis in Human Mesenchymal Progenitor Cells Via a Hedgehog Dependent Mechanism.

Neelima Thottappillil, Mario A Gomez-Salazar, Mingxin Xu, Qizhi Qin, Xin Xing, Jiajia Xu, Kristen Broderick, Ji-Hye Yea, Mary Archer, Ginny Ching-Yun Hsu and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Neelima ThottappillilDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.ORCID 0000-0003-3973-7117
Mario A Gomez-SalazarDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Mingxin XuDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Qizhi QinDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Xin XingDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Jiajia XuDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Kristen BroderickDepartment of Plastic Surgery, Johns Hopkins University, Baltimore, MD, USA.
Ji-Hye YeaDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Mary ArcherDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Ginny Ching-Yun HsuDepartment of Orthodontics, Oregon Health and Science University, Portland, OR, USA.
Bruno PéaultDepartment of Orthopaedic Surgery and Orthopaedic Hospital Research Center, UCLA, Los Angeles, CA, USA.
Aaron W JamesDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Johns Hopkins University · USOregon Health & Science University · USOrthopaedic Hospital · US

Funding

NELL-1 Systemic Therapy for OsteoporosisR01AR066782 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI EASLEY, JEREMIAH, TING, KANG · 2014 to 2018
$2.0M
Direct and indirect contributions of perivascular stem cells to bone healingR01AR070773 · NIAMS · JOHNS HOPKINS UNIVERSITY · PI JAMES, AARON W · 2017 to 2021
$1.4M
TIAM1 dictates lineage commitment in skeletal and soft tissue pericytesK08DE031347 · NIDCR · OREGON HEALTH & SCIENCE UNIVERSITY · PI Ginny Ching-Yun Hsu · 2022 to 2026
$830k
NELL-1 isoforms for the systemic treatment of osteoporosisK08AR068316 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JAMES, AARON W · 2015 to 2019
$534k
NIAMS NIH HHS K08 AR068316NIAMS NIH HHS R01 AR066782NIAMS NIH HHS R01 AR070773NIDCR NIH HHS K08 DE031347
6 · The paper itself

Abstract

Numerous intrinsic factors regulate mesenchymal progenitor commitment to a specific cell fate, such as osteogenic or adipogenic lineages. Identification and modulation of novel intrinsic regulatory factors represent an opportunity to harness the regenerative potential of mesenchymal progenitors. In the present study, the transcription factor (TF) ZIC1 was identified to be differentially expressed among adipose compared with skeletal-derived mesenchymal progenitor cells. We observed that ZIC1 overexpression in human mesenchymal progenitors promotes osteogenesis and prevents adipogenesis. ZIC1 knockdown demonstrated the converse effects on cell differentiation. ZIC1 misexpression was associated with altered Hedgehog signaling, and the Hedgehog antagonist cyclopamine reversed the osteo/adipogenic differentiation alterations associated with ZIC1 overexpression. Finally, human mesenchymal progenitor cells with or without ZIC1 overexpression were implanted in an ossicle assay in NOD-SCID gamma mice. ZIC1 overexpression led to significantly increased ossicle formation in comparison to the control, as assessed by radiographic and histologic measures. Together, these data suggest that ZIC1 represents a TF at the center of osteo/adipogenic cell fate determinations-findings that have relevance in the fields of stem cell biology and therapeutic regenerative medicine.

Indexed as

AdipogenesisMesenchymal Stem CellsAnimalsCell DifferentiationHedgehog ProteinsHumansMiceMice, Inbred NODMice, SCIDOsteogenesisTranscription FactorsHedgehog ProteinsTranscription FactorsZIC1 protein, humanadipogenesisbone tissue engineeringmesenchymal progenitor cellsmesenchymal stem cellossicle formationosteogenesispericytesSonic Hedgehog signalingZIC1

Identifiers

PMID37317792
PMCPMC10502786
OpenAlexW4380669169

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.