Evidence map›Paper›PMID 37316783›Full record

ArticleBMC plant biology2023

Melatonin strongly enhances the Agrobacterium- mediated transformation of carnation in nitrogen-depleted media.

Omid Aalami, Pejman Azadi, Hanieh Hadizadeh, H Dayton Wilde, Zahra Karimian, Hossein Nemati, Leila Samiei

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Omid AalamiResearch Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, 91779948974, Iran.
Pejman AzadiDepartment of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.
Hanieh HadizadehResearch Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, 91779948974, Iran.
H Dayton WildeHorticulture Department, University of Georgia, Athens, GA, USA.
Zahra KarimianResearch Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, 91779948974, Iran.
Hossein NematiDepartment of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, 91779948974, Iran.
Leila SamieiResearch Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, 91779948974, Iran. samiei@um.ac.ir.
Ferdowsi University of Mashhad · IRAgricultural Biotechnology Research Institute of Iran · IRUniversity of Georgia · US

Funding

Ferdowsi University of Mashhad 47719
6 · The paper itself

Abstract

With the rising demand for new cultivars of carnation, efficient transformation protocols are needed to enable the bioengineering of new traits. Here, we established a novel and efficient Agrobacterium-mediated transformation system using callus as the target explant for four commercial carnation cultivars. Leaf-derived calli of all cultivars were inoculated with Agrobacterium tumefaciens strain LBA4404 containing the plasmid pCAMBIA 2301 harboring genes for β-glucuronidase (uidA) and neomycin phosphotransferase (nptII). Polymerase chain reaction (PCR) and histochemical assays confirmed the presence of uidA and β-glucuronidase (GUS), respectively in transgenic shoots. The effect on transformation efficiency of medium composition and the presence of antioxidants during inoculation and co-cultivation was investigated. The transformation efficiency was increased in Murashige and Skoog (MS) medium lacking KNO

Indexed as

DianthusMelatoninAgrobacterium tumefaciensGlucuronidaseNitrogenGlucuronidaseMelatoninNitrogenAgrobacterium tumefaciensAntioxidant compoundsDianthus caryophyllusTransformation efficiencyα-lipoic acid

Identifiers

PMID37316783
PMCPMC10265774
OpenAlexW4380442277

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.