Evidence map›Paper›PMID 37316758›Full record

ArticleMolecular neurobiology2023

The Temporal and Spatial Changes of Autophagy and PI3K Isoforms in Different Neural Cells After Hypoxia/Reoxygenation Injury.

Duo Zhang, Xuanyu Chen, Baoge Liu, Yuan Yuan, Wei Cui, Di Zhu, Jichao Zhu, Shuo Duan, Chenxi Li

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular neurobiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Duo ZhangDepartment of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China.
Xuanyu ChenDepartment of Orthopedics, Capital Medical University Electric Power Hospital, Beijing, 100073, China.
Baoge LiuDepartment of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China. baogeliu@hotmail.com.ORCID http://orcid.org/0000-0002-2156-0870
Yuan YuanDepartment of Spinal Cord Injury Rehabilitation, China Rehabilitation Research Center, Beijing, 100068, China.
Wei CuiDepartment of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China.
Di ZhuDepartment of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China.
Jichao ZhuDepartment of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China.
Shuo DuanDepartment of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China.
Chenxi LiDepartment of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China.

Funding

Beijing Excellent Talent Training Funding 2017000021469G215the National Key Research and Development Program of China 2018YFF0301103the Natural Science Foundation of Capital Medical University of China PYZ2017082the Natural Science Foundation of Capital Medical University of China PYZ2018081
6 · The paper itself

Abstract

There are limited therapeutic options for patient with traumatic spinal cord injury (SCI). Phosphoinositide 3-kinase family (PI3Ks) are the key molecules for regulating cell autophagy, which is a possible way of treating SCI. As we know, PI3K family are composed of eight isoforms, which are distributed into three classes. While the role of PI3Ks in regulating autophagy is controversial and the effects may be in a cell-specific manner. Different isoforms do not distribute in neural cells consistently and it is not clear how the PI3K isoforms regulate and interact with autophagy. Therefore, we explored the distributions and expression of different PI3K isoforms in two key neural cells (PC12 cells and astrocytes). The results showed that the expression of LC3II/I and p62, which are the markers of autophagy, changed in different patterns in PC12 cells and astrocytes after hypoxia/reoxygenation injury (H/R). Furthermore, the mRNA level of eight PI3K isoforms did not change in the same way, and even for the same isoform the mRNA activities are different between PC12 cells and astrocytes. What is more, the results of western blot of PI3K isoforms after H/R were inconsistent with the relevant mRNA. Based on this study, the therapeutic effects of regulating autophagy on SCI are not confirmed definitely, and its molecular mechanisms may be related with different temporal and spatial patterns of activation and distributions of PI3K isoforms.

Indexed as

AutophagyPhosphatidylinositol 3-KinasesAnimalsApoptosisHumansHypoxiaPhosphatidylinositol 3-KinaseRatsRNA, MessengerPhosphatidylinositol 3-KinasePhosphatidylinositol 3-KinasesRNA, MessengerAstrocytesAutophagyHypoxia/reoxygenation injuryPC12 cellsPI3K isoformsSpinal cord injury

Identifiers

PMID37316758

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.