Evidence map›Paper›PMID 37314924›Full record

ArticleSTAR protocols2023

Protocol for purification of cells in their native state using reversible aptamer-antidote pairs.

Martin D Requena, Bethany Powell Gray, Bruce A Sullenger

Open access · goldAbstract readComment
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Martin D RequenaDepartment of Surgery, Duke University Medical Center, 2 Genome Ct, Durham, NC 27710, USA; University Program in Genetics and Genomics, Duke University Medical Center, 2 Genome Ct, Durham, NC 27710, USA. Electronic address: mdr45@duke.edu.
Bethany Powell GrayDepartment of Surgery, Duke University Medical Center, 2 Genome Ct, Durham, NC 27710, USA; Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, 725 North Wolfe St, Baltimore, MD 21205, USA.
Bruce A SullengerDepartment of Surgery, Duke University Medical Center, 2 Genome Ct, Durham, NC 27710, USA; University Program in Genetics and Genomics, Duke University Medical Center, 2 Genome Ct, Durham, NC 27710, USA. Electronic address: bruce.sullenger@duke.edu.
Duke University · USJohns Hopkins University · US

Funding

Translating Mechanistic Insights into Intrinsic Xase FunctionP01HL139420 · NHLBI · CHILDREN'S HOSP OF PHILADELPHIA · PI Sriram Krishnaswamy · 2018 to 2026
$22.4M
Direct Detection and Characterization of Thrombosis In VivoR01HL147147 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SULLENGER, BRUCE ALAN, TARANTAL, ALICE F · 2019 to 2022
$3.0M
NHLBI NIH HHS P01 HL139420NHLBI NIH HHS R01 HL147147
6 · The paper itself

Abstract

Cell isolation from complex mixtures is a key step in many clinical and research applications, but standard isolation methods may affect the cell's biology and are difficult to reverse. Here, we present a method to isolate and restore cells to their native state using an aptamer that binds epidermal growth factor receptor (EGFR+)cells and a complementary antisense oligonucleotide to reverse binding. For complete details on the use and execution of this protocol, please refer to Gray et al.

Indexed as

AntidotesOligonucleotidesCell SeparationOligonucleotides, AntisenseAntidotesOligonucleotidesOligonucleotides, AntisenseBiotechnology and bioengineeringCell BiologyCell isolationMolecular/Chemical Probes

Identifiers

PMID37314924
PMCPMC10277588
OpenAlexW4380730233

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.