ArticleMolecular breeding : new strategies in plant improvement2023
Development of SNP marker panels for genotyping by target sequencing (GBTS) and its application in soybean.
Article in Molecular breeding : new strategies in plant improvement, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 17 citations in OpenAlex.
- Development and application of a 5K targeted liquid SNP panel for Hetian chickens based on genotyping by targeted sequencing.Poultry science · 2026Article
- Development of a Medium-Density Genotyping Platform to Accelerate Genetic Gain in Fresh Edible Maize.Plants (Basel, Switzerland) · 2026Article
- Development of liquid chip for functional genes in foxtail millet (Frontiers in plant science · 2026Article
- Genome-Wide Association Study Dissects the Genetic Architecture of Pericarp Traits in Fresh-Eating Maize.Plants (Basel, Switzerland) · 2025Article
- Development of SNP panel for genetic diversity assessment, fingerprinting identification and backcross breeding inMolecular breeding : new strategies in plant improvement · 2025Article
- Design, validation, and application of a 1K liquid chip for genome-wide association analysis in Alpine Merino sheep.Frontiers in veterinary science · 2025Article
- Allele-tagged TaqManMolecular biology reports · 2024Article
- Identification ofPlants (Basel, Switzerland) · 2024Article
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- Development of a 45K pepper GBTS liquid-phase gene chip and its application in genome-wide association studies.Frontiers in plant science · 2024Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A high-throughput genotyping platform with customized flexibility, high genotyping accuracy, and low cost is critical for marker-assisted selection and genetic mapping in soybean. Three assay panels were selected from the SoySNP50K, 40K, 20K, and 10K arrays, containing 41,541, 20,748, and 9670 SNP markers, respectively, for genotyping by target sequencing (GBTS). Fifteen representative accessions were used to assess the accuracy and consistency of the SNP alleles identified by the SNP panels and sequencing platform. The SNP alleles were 99.87% identical between technical replicates and 98.86% identical between the 40K SNP GBTS panel and 10× resequencing analysis. The GBTS method was also accurate in the sense that the genotypic dataset of the 15 representative accessions correctly revealed the pedigree of the accessions, and the biparental progeny datasets correctly constructed the linkage maps of the SNPs. The 10K panel was also used to genotype two parent-derived populations and analyze QTLs controlling 100-seed weight, resulting in the identification of the stable associated genetic locus Supplementary Information: The online version contains supplementary material available at 10.1007/s11032-023-01372-6.
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