Evidence map›Paper›PMID 37313021›Full record

ArticleBasic and clinical neuroscience

Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture.

Ardeshir Moayeri, Rafieh Alizadeh, Hatef Ghasemi Hamidabadi, Maryam Nazm Bojnordi, Ali Niapour, Azim Hedayatpour, Marzieh Darvishi, Fatemeh Heidari, Maryam Soleimani, Leila Elyasi

Abstract read
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In one paragraph

Article in Basic and clinical neuroscience. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Research Progress on Neural Cell Culture Systems.Current neuropharmacology · 2025
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ardeshir MoayeriDepartment of Anatomy, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.ORCID https://orcid.org/0000-0002-3704-5347
Rafieh AlizadehENT and Head and Neck Research Center and Department, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.ORCID https://orcid.org/0000-0002-3132-9904
Hatef Ghasemi HamidabadiDepartment of Anatomy & Cell Biology, Immunogenetic Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.ORCID https://orcid.org/0000-0002-2178-6503
Maryam Nazm BojnordiDepartment of Anatomy & Cell Biology, Immunogenetic Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.ORCID https://orcid.org/0000-0003-1176-2417
Ali NiapourDepartment of Anatomical Sciences, Research Laboratory for Embryology and Stem Cells, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.ORCID https://orcid.org/0000-0002-9013-4664
Azim HedayatpourDepartment of Anatomy, School of Medicine, University of Tehran, Tehran, Iran.ORCID https://orcid.org/0000-0002-1036-0697
Marzieh DarvishiDepartment of Anatomy, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.ORCID https://orcid.org/0000-0002-5004-6213
Fatemeh HeidariDepartment of Anatomy, School of Medicine, Qom University of Medical Sciences, Qom, Iran.ORCID https://orcid.org/0000-0003-3822-3480
Maryam SoleimaniDepartment of Basic Sciences, School of Rehabilitation Sciences, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.ORCID https://orcid.org/0000-0003-2650-9174
Leila ElyasiDepartment of Anatomy, Neuroscience Research Center, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.ORCID https://orcid.org/0000-0002-2618-2434

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: The induction of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in tissue engineering and experimental and clinical treatments of various neurodegenerative diseases, including Parkinson disease. This study aims to differentiate HUC-MSCs into dopaminergic neuron-like cells. Methods: Following the isolation and characterization of HUC-MSCs, they were transferred to Matrigel-coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuron-like cells in 2-dimensional culture and on Matrigel was assessed by real-time polymerase chain reaction, immunocytochemistry, and high-performance liquid chromatography. Results: Our results showed that dopaminergic neuronal markers' transcript and protein levels were significantly increased on the Matrigel differentiated cells compared to 2D culture plates. Conclusion: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potential for the treatment of dopaminergic neuron-related diseases.

Indexed as

DifferentiationDopaminergic neuronsHuman umbilical cord mesenchymal stem cellsIn vitro

Identifiers

PMID37313021
PMCPMC10258594

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.