Evidence map›Paper›PMID 37312651›Full record

ArticleProtein science : a publication of the Protein Society2023

A disulfide chaperone knockout facilitates spin labeling and pulse EPR spectroscopy of outer membrane transporters.

Viranga W Wimalasiri, Kinga A Jurczak, Monika K Wieliniec, Thushani D Nilaweera, Robert K Nakamoto, David S Cafiso

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein science : a publication of the Protein Society, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Viranga W WimalasiriDepartment of Chemistry and Center for Membrane Biology, University of Virginia, Charlottesville, Virginia, USA.
Kinga A JurczakDepartment of Chemistry and Center for Membrane Biology, University of Virginia, Charlottesville, Virginia, USA.
Monika K WieliniecDepartment of Chemistry and Center for Membrane Biology, University of Virginia, Charlottesville, Virginia, USA.
Thushani D NilaweeraDepartment of Chemistry and Center for Membrane Biology, University of Virginia, Charlottesville, Virginia, USA.
Robert K NakamotoDepartment of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA.
David S CafisoDepartment of Chemistry and Center for Membrane Biology, University of Virginia, Charlottesville, Virginia, USA.ORCID 0000-0002-3813-8721
University of Virginia · USNational Institutes of Health · US

Funding

MOLECULAR MECHANISMS OF MEMBRANE TRANSPORTR01GM035215 · NIGMS · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI DAVID S CAFISO · 1985 to 2026
$7.3M
Upgrade of Bruker EPR SpectrometerS10OD025149 · OD · UNIVERSITY OF VIRGINIA · PI CAFISO, DAVID S · 2018 to 2018
$396k
NIGMS NIH HHS R01 GM035215NIH HHS S10 OD025149
6 · The paper itself

Abstract

Pulse EPR measurements provide information on distances and distance distributions in proteins but require the incorporation of pairs of spin labels that are usually attached to engineered cysteine residues. In previous work, we demonstrated that efficient in vivo labeling of the Escherichia coli outer membrane vitamin B

Indexed as

Escherichia coli ProteinsMembrane Transport ProteinsBacterial Outer Membrane ProteinsDisulfidesElectron Spin Resonance SpectroscopyEscherichia coliMolecular ChaperonesReceptors, Cell SurfaceSpin LabelsBacterial Outer Membrane ProteinsDisulfidesEscherichia coli ProteinsFecA protein, E coliMembrane Transport ProteinsMolecular ChaperonesReceptors, Cell SurfaceSpin Labelsin vivo EPR spectroscopyouter membrane proteinssite-directed spin-labelingTonB-dependent transport

Identifiers

PMID37312651
PMCPMC10288552
OpenAlexW4380576591

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.