Evidence map›Paper›PMID 37311907›Full record

ArticleJournal of biomolecular NMR2023

E. coli "Stablelabel" S30 lysate for optimized cell-free NMR sample preparation.

Roman Levin, Frank Löhr, Betül Karakoc, Roman Lichtenecker, Volker Dötsch, Frank Bernhard

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomolecular NMR, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Roman LevinInstitute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438, Frankfurt, Germany.ORCID https://orcid.org/0000-0002-0323-4086
Frank LöhrInstitute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438, Frankfurt, Germany.
Betül KarakocInstitute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438, Frankfurt, Germany.
Roman LichteneckerInstitute of Organic Chemistry, University of Vienna, 1090, Vienna, Austria.
Volker DötschInstitute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438, Frankfurt, Germany.ORCID https://orcid.org/0000-0001-5720-212X
Frank BernhardInstitute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438, Frankfurt, Germany. fbern@bpc.uni-frankfurt.de.ORCID https://orcid.org/0000-0002-4860-1783

Funding

Center for Biomolecular Magnetic Resonance BMRZDeutsche Forschungsgemeinschaft BE1911/8-1LOEWE GLUE
6 · The paper itself

Abstract

Cell-free (CF) synthesis with highly productive E. coli lysates is a convenient method to produce labeled proteins for NMR studies. Despite reduced metabolic activity in CF lysates, a certain scrambling of supplied isotope labels is still notable. Most problematic are conversions of

Indexed as

Amino AcidsEscherichia coliCell-Free SystemIsotope LabelingNuclear Magnetic Resonance, BiomolecularProtein BiosynthesisProteinsAmino AcidsProteinsCell-free expressionMetabolic engineeringNMR of membrane proteinsProtein labelingS30 lysateStable isotope scrambling

Identifiers

PMID37311907
PMCPMC10406690

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.