Evidence map›Paper›PMID 37310631›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

In Vitro Culture and Histological Evaluation of 3D Organotypic Cultures.

William Dalleywater, Francesca Wheat, Declan Sculthorpe, Georgina Hyland, Mohammad Ilyas

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

William DalleywaterTranslational Medical Sciences, School of Medicine, University of Nottingham, Nottingham, UK. William.dalleywater@nottingham.ac.uk.
Francesca WheatDepartment of Cellular Pathology, University Hospitals of Leicester NHS Trust, Nottingham, UK.
Declan SculthorpeTranslational Medical Sciences, School of Medicine, University of Nottingham, Nottingham, UK.
Georgina HylandTranslational Medical Sciences, School of Medicine, University of Nottingham, Nottingham, UK.
Mohammad IlyasTranslational Medical Sciences, School of Medicine, University of Nottingham, Nottingham, UK.

Funding

Medical Research Council MR/R017336/1
6 · The paper itself

Abstract

Organotypic cultures allow cells to grow in a system which mimics in vivo tissue organization. Here we describe a method for establishing 3D organotypic cultures (using intestine as an example system), followed by methods for demonstrating cell morphology and tissue architecture using histological techniques and molecular expression analysis using immunohistochemistry, though the system is also amenable to molecular expression analysis, such as by PCR, RNA sequencing, or FISH.

Indexed as

Histological TechniquesMicrophysiological SystemsPolymerase Chain Reaction3D organotypic culturesImmunohistochemistryIntestineIn vitro

Identifiers

PMID37310631

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.