ArticlePlant & cell physiology2023
A Stereoselective Strigolactone Biosynthesis Catalyzed by a 2-Oxoglutarate-Dependent Dioxygenase in Sorghum.
Article in Plant & cell physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 21 citations in OpenAlex.
- Is resistance ever free? Weighing the costs of altered strigolactone biosynthesis in sorghum.Plant physiology · 2026Article
- Stereospecific Antifungal Activity of Strigolactone Analogues AgainstJournal of fungi (Basel, Switzerland) · 2026Article
- A powerful molecular marker to detect mutations at sorghum LOW GERMINATION STIMULANT 1.The plant genome · 2025Article
- Genome-wide identification and comparative analysis of strigolactone biosynthetic genes in major solanaceous crops.PloS one · 2025Article
- Insights into stereoselective ring formation in canonical strigolactone: Identification of a dirigent domain-containing enzyme catalyzing orobanchol synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- 2-oxoglutarate-dependent dioxygenases and BAHD acyltransferases drive the structural diversification of orobanchol in Fabaceae plants.Frontiers in plant science · 2024Article
- Strigolactones and Shoot Branching: What Is the Real Hormone and How Does It Work?Plant & cell physiology · 2023Review
- Biosynthetic Pathways of Hormones in Plants.Metabolites · 2023Review
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Seeds of root parasitic plants, Striga, Orobanche and Phelipanche spp., are induced to germinate by strigolactones (SLs) exudated from host roots. In Striga-resistant cultivars of Sorghum bicolor, the loss-of-function of the Low Germination Stimulant 1 (LGS1) gene changes the major SL from 5-deoxystrigol (5DS) to orobanchol, which has an opposite C-ring stereochemistry. The biosynthetic pathway of 5DS catalyzed by LGS1 has not been fully elucidated. Since other unknown regulators, in addition to LGS1 encoding a sulfotransferase, appear to be necessary for the stereoselective biosynthesis of 5DS, we examined Sobic.005G213500 (Sb3500), encoding a 2-oxoglutarate-dependent dioxygenase, as a candidate regulator, which is co-expressed with LGS1 and located 5'-upstream of LGS1 in the sorghum genome. When LGS1 was expressed with known SL biosynthetic enzyme genes including the cytochrome P450 SbMAX1a in Nicotiana benthamiana leaves, 5DS and its diastereomer 4-deoxyorobanchol (4DO) were produced in approximately equal amounts, while the production of 5DS was significantly larger than that of 4DO when Sb3500 was also co-expressed. We also confirmed the stereoselective 5DS production in an in vitro feeding experiment using synthetic chemicals with recombinant proteins expressed in Escherichia coli and yeast. This finding demonstrates that Sb3500 is a stereoselective regulator in the conversion of the SL precursor carlactone to 5DS, catalyzed by LGS1 and SbMAX1a, providing a detailed understanding of how different SLs are produced to combat parasitic weed infestations.
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