Evidence map›Paper›PMID 37306831›Full record

ArticleJournal of microbiology (Seoul, Korea)2023

Heterologous Production and Structure Determination of a New Lanthipeptide Sinosporapeptin Using a Cryptic Gene Cluster in an Actinobacterium Sinosporangium siamense.

Keita Saito, Keiichiro Mukai, Issara Kaweewan, Hiroyuki Nakagawa, Takeshi Hosaka, Shinya Kodani

Abstract read
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In one paragraph

Article in Journal of microbiology (Seoul, Korea), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.9field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Keita SaitoGraduate School of Integrated Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan.
Keiichiro MukaiGraduate School of Medicine, Science and Technology, Shinshu University, Nagano, 399-4598, Japan.
Issara KaweewanFaculty of Agriculture, Shizuoka University, Shizuoka, 422-8529, Japan.
Hiroyuki NakagawaResearch Center for Advanced Analysis, Core Technology Research Headquarters, National Agriculture and Food Research Organization (NARO), Ibaraki, 305-8642, Japan.
Takeshi HosakaGraduate School of Medicine, Science and Technology, Shinshu University, Nagano, 399-4598, Japan.
Shinya KodaniGraduate School of Integrated Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan. kodani.shinya@shizuoka.ac.jp.ORCID http://orcid.org/0000-0002-6792-1184
Shizuoka University · JPShinshu University · JPNational Agriculture and Food Research Organization · JP

Funding

Institute for Fermentation, Osaka G-2021-3-010JSPS 20K05848JSPS 21F21095Kobayashi Foundation 196
6 · The paper itself

Abstract

Lipolanthine is a subclass of lanthipeptide that has the modification of lipid moiety at the N-terminus. A cryptic biosynthetic gene cluster comprising four genes (sinA, sinKC, sinD, and sinE) involved in the biosynthesis of lipolanthine was identified in the genome of an actinobacterium Sinosporangium siamense. Heterologous coexpression of a precursor peptide coding gene sinA and lanthipeptide synthetase coding gene sinKC in the host Escherichia coli strain BL21(DE3) resulted in the synthesis of a new lanthipeptide, sinosporapeptin. It contained unusual amino acids, including one labionin and two dehydrobutyrine residues, as determined using NMR and MS analyses. Another coexpression experiment with two additional genes of decarboxylase (sinD) and N-acetyl transferase (sinE) resulted in the production of a lipolanthine-like modified sinosporapeptin.

Indexed as

ActinobacteriaMultigene FamilyPeptidesAmino AcidsAnti-Bacterial AgentsBacteriaEscherichia coliGenes, BacterialModels, MolecularProtein Structure, TertiaryTranscriptomeAmino AcidsAnti-Bacterial AgentsPeptidesBiosynthesisHeterologous expressionLanthipeptideLipolanthine

Identifiers

PMID37306831
OpenAlexW4380291692

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.