Evidence map›Paper›PMID 37306827›Full record

ArticleBiochemical genetics2024

FOXC1 Promotes Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells via the Dnmt3b/CXCL12 Axis.

Peiguang Zhang, Bo Feng, Guangming Dai, Kecheng Niu, Lan Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Biochemical genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Peiguang ZhangDepartment of Orthopedics, The Third Affiliated Hospital, Inner Mongolia Medical University, No. 20 Shaoxian Road, Kundulun District, Baotou, 014010, Inner Mongolia, People's Republic of China.
Bo FengDepartment of Orthopedics, The Third Affiliated Hospital, Inner Mongolia Medical University, No. 20 Shaoxian Road, Kundulun District, Baotou, 014010, Inner Mongolia, People's Republic of China.
Guangming DaiDepartment of Orthopedics, The Third Affiliated Hospital, Inner Mongolia Medical University, No. 20 Shaoxian Road, Kundulun District, Baotou, 014010, Inner Mongolia, People's Republic of China.
Kecheng NiuDepartment of Orthopedics, The Third Affiliated Hospital, Inner Mongolia Medical University, No. 20 Shaoxian Road, Kundulun District, Baotou, 014010, Inner Mongolia, People's Republic of China.
Lan ZhangDepartment of Orthopedics, The Third Affiliated Hospital, Inner Mongolia Medical University, No. 20 Shaoxian Road, Kundulun District, Baotou, 014010, Inner Mongolia, People's Republic of China. lanzhang19888@163.com.
Inner Mongolia Medical University · CNThird Affiliated Hospital of Inner Mongolia Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone defects have remained a clinical problem in current orthopedics. Bone marrow mesenchymal stem cells (BM-MSCs) with multi-directional differentiation ability have become a research hotspot for repairing bone defects. In vitro and in vivo models were constructed, respectively. Alkaline phosphatase (ALP) staining and alizarin red staining were performed to detect osteogenic differentiation ability. Western blotting (WB) was used to detect the expression of osteogenic differentiation-related proteins. Serum inflammatory cytokine levels were detected by ELISA. Fracture recovery was evaluated by HE staining. The binding relationship between FOXC1 and Dnmt3b was verified by dual-luciferase reporter assay. The relationship between Dnmt3b and CXCL12 was explored by MSP and ChIP assays. FOXC1 overexpression promoted calcium nodule formation, upregulated osteogenic differentiation-related protein expression, promoted osteogenic differentiation, and decreased inflammatory factor levels in BM-MSCs, and promoted callus formation, upregulated osteogenic differentiation-related protein expression, and downregulated CXCL12 expression in the mouse model. Furthermore, FOXC1 targeted Dnmt3b, with Dnmt3b knockdown decreasing calcium nodule formation and downregulating osteogenic differentiation-related protein expression. Additionally, inhibiting Dnmt3b expression upregulated CXCL12 protein expression and inhibited CXCL12 methylation. Dnmt3b could be binded to CXCL12. CXCL12 overexpression attenuated the effects of FOXC1 overexpression and inhibited BM-MSCs osteogenic differentiation. This study confirmed that the FOXC1-mediated regulation of the Dnmt3b/CXCL12 axis had positive effects on the osteogenic differentiation of BM-MSCs.

Indexed as

Mesenchymal Stem CellsMicroRNAsAnimalsCalciumCell DifferentiationCells, CulturedCytokinesMiceOsteogenesisCalciumCytokinesMicroRNAsBM-MSCsCXCL12Dnmt3bFOXC1Osteoblastic differentiation

Identifiers

PMID37306827
OpenAlexW4380290921

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.