Evidence map›Paper›PMID 37306707›Full record

ArticleApplied microbiology and biotechnology2023

Quickly assessing disinfection effectiveness to control the spread of African swine fever virus.

Dexin Zeng, Bingxu Qian, Yunfei Li, Kai Zong, Liu Ding, Manman Wang, Tingting Zhou, Xiaying Lv, Kun Zhu, Xiaofeng Yu and 4 more

Abstract read
PubMed Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Dexin Zeng *National Key Laboratory of Meat Quality Control and New Resource Creation, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Bingxu Qian *National Key Laboratory of Meat Quality Control and New Resource Creation, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Yunfei LiTechnical Center of Hefei Customs, Hefei, 230022, People's Republic of China.
Kai ZongTechnical Center of Hefei Customs, Hefei, 230022, People's Republic of China.
Liu DingTechnical Center of Hefei Customs, Hefei, 230022, People's Republic of China.
Manman WangTechnical Center of Hefei Customs, Hefei, 230022, People's Republic of China.
Tingting ZhouTechnical Center of Hefei Customs, Hefei, 230022, People's Republic of China.
Xiaying LvTechnical Center of Hefei Customs, Hefei, 230022, People's Republic of China.
Kun ZhuBeiJing OriginGene-Tech Biotechnology Co., Ltd, Beijing, 100176, People's Republic of China.
Xiaofeng YuTechnical Center of Hefei Customs, Hefei, 230022, People's Republic of China.
Yuan JiangAnimal, Plant and Food Inspection Center of Nanjing Customs, Nanjing, 210095, People's Republic of China.
Xiaodong WuChina Animal Health and Epidemiology Center, Qingdao, Shandong, 266032, People's Republic of China. wuxiaodong@cahec.cn.
Feng XueNational Key Laboratory of Meat Quality Control and New Resource Creation, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. xuefeng@njau.edu.cn.ORCID http://orcid.org/0000-0002-2437-9858
Jianjun DaiNational Key Laboratory of Meat Quality Control and New Resource Creation, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

Funding

Hainan Province Scicence and Technology Special Fund ZDYF2022XDNY248Jiangsu Agricultural Science and Technology Independent Innovation Fund Project CX (21) 2038National Key Research and Development Program of China 2021YFD1800500Sanya Nanjing Agricultural University Research Institute Guiding Fund Project NAUSY-ZD08
6 · The paper itself

Abstract

Infectious African swine fever virus (ASFV) can cause the spread and morbidity of African swine fever, while the inactivated virus cannot. When they are not distinguished separately, the detection results will lack authenticity and cause unnecessary panic and detection cost. The detection technology based on cell culture is complex, high-cost, and time-consuming in practice, which is not conducive to the rapid detection of infectious ASFV. In this study, a propidium monoazide (PMA) qPCR detection method for rapid diagnosis of infectious ASFV was constructed. Parameters of PMA concentration, light intensity, and lighting time were under strict safety verification and comparative analysis for optimization. The results determined that the optimal condition for PMA to pretreat ASFV was the final concentration of PMA 100 μM. The light intensity was 40 W, the light duration was 20 min, the target fragment size of the optimal primer probe was 484 bp, and its detection sensitivity for infectious ASFV was 10

Indexed as

African Swine FeverAfrican Swine Fever VirusDisinfectantsAnimalsChlorineDisinfectionSwineChlorineDisinfectantsASFASFVEvaluation of disinfection effectInfectious virusPMA

Identifiers

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.