Evidence map›Paper›PMID 37306697›Full record

ArticleAdvances in biochemical engineering/biotechnology2023

Cell-Free Display Techniques for Protein Evolution.

Jiaojiao Li, Youhui Yang, Jinjin Li, Peixian Li, Hao Qi

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Article in Advances in biochemical engineering/biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. In Vitro Selection of Antibodies by Ribosome Display.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jiaojiao LiSchool of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Youhui YangSchool of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Jinjin LiSchool of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Peixian LiSchool of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Hao QiSchool of Chemical Engineering and Technology, Tianjin University, Tianjin, China. haoq@tju.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-free protein synthesis (CFPS) with flexibility and controllability can provide a powerful platform for high-throughput screening of biomolecules, especially in the evolution of peptides or proteins. In this chapter, the emerging strategies for enhancing the protein expression level using different source strains, energy systems, and template designs in constructing CFPS systems are summarized and discussed in detail. In addition, we provide an overview of the ribosome display, mRNA display, cDNA display, and CIS display in vitro display technologies, which can couple genotype and phenotype by forming fusion complexes. Moreover, we point out the trend that improving the protein yields of CFPS itself can offer more favorable conditions for maintaining library diversity and display efficiency. It is hoped that the novel CFPS system can accelerate the development of protein evolution in biotechnological and medical applications.

Indexed as

ProteinsRibosomesCell-Free SystemDNA, ComplementaryGene LibraryProtein BiosynthesisDNA, ComplementaryProteinscDNA displayCell-free systemCIS displaymRNA displayProtein evolutionRibosome display

Identifiers

PMID37306697

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.