Evidence map›Paper›PMID 37306605›Full record

ArticleMicrobiology spectrum2023

Detection and Quantification of Klebsiella pneumoniae in Fecal Samples Using Digital Droplet PCR in Comparison with Real-Time PCR.

Junxia Feng, Xiaohu Cui, Bing Du, Hanqing Zhao, Yanling Feng, Jinghua Cui, Chao Yan, Lin Gan, Zheng Fan, Tongtong Fu and 8 more

Abstract read
In one paragraph

Article in Microbiology spectrum, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
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  4. Article
  5. Article
  6. Recent advances in diagnostic technologies for postoperative central nervous system infections: a review.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2025
    Review
  7. Article
  8. Review
  9. Detection ofPathogens (Basel, Switzerland) · 2025
    Article
  10. Article
  11. Article
  12. Article
  13. Multiplex droplet digital PCR for the detection and quantitation ofFrontiers in cellular and infection microbiology · 2025
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Junxia Feng *Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Xiaohu Cui *Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Bing DuDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Hanqing ZhaoDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Yanling FengDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Jinghua CuiDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Chao YanDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Lin GanDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Zheng FanDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Tongtong FuDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Ziying XuDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Zihui YuDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Rui ZhangDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Shuheng DuDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Ziyan TianDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Qun ZhangDepartment of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Guanhua Xue *Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
Jing Yuan *Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China.ORCID 0000-0002-6939-9676

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to develop a rapid and sensitive droplet digital PCR (ddPCR) assay for the specific detection of Klebsiella pneumoniae in fecal samples, and to evaluate its application in the clinic by comparison with real-time PCR assay and conventional microbial culture. Specific primers and a probe targeting the K. pneumoniae hemolysin (

Indexed as

Klebsiella pneumoniaeFecesHumansReal-Time Polymerase Chain ReactionReproducibility of ResultsSensitivity and Specificitydroplet digital PCRKlebsiella pneumoniaequantificationreal-time PCR

Identifiers

PMID37306605
PMCPMC10433836

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.