ArticleBiophysical journal1986
Protein rotational motion in solution measured by polarized fluorescence depletion.
Article in Biophysical journal, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Continuous Fluorescence Depletion Anisotropy Measurement of Protein Rotation.Journal of fluorescence · 2018Article
- Fluorescence lifetime excitation cytometry by kinetic dithering.Electrophoresis · 2014Article
- Measurement of single macromolecule orientation by total internal reflection fluorescence polarization microscopy.Biophysical journal · 2005Article
- Polarized fluorescence depletion reports orientation distribution and rotational dynamics of muscle cross-bridges.Biophysical journal · 2002Article
- End-to-end diffusion on the microsecond timescale measured with resonance energy transfer from a long-lifetime rhenium metal-ligand complex.Photochemistry and photobiology · 2000Article
- Cytoplasmic viscosity near the cell plasma membrane: translational diffusion of a small fluorescent solute measured by total internal reflection-fluorescence photobleaching recovery.Biophysical journal · 1996Article
- Control of band 3 lateral and rotational mobility by band 4.2 in intact erythrocytes: release of band 3 oligomers from low-affinity binding sites.Biophysical journal · 1996Article
- Myosin head rotation in muscle fibers measured using polarized fluorescence photobleaching recovery.Journal of fluorescence · 1995Article
- Subnanosecond polarized fluorescence photobleaching: rotational diffusion of acetylcholine receptors on developing muscle cells.Biophysical journal · 1995Article
- Differential control of band 3 lateral and rotational mobility in intact red cells.The Journal of clinical investigation · 1994Article
- Photobleaching with a subnanosecond laser flash.Journal of fluorescence · 1994Article
- Duplication of 10 nucleotides in the erythroid band 3 (AE1) gene in a kindred with hereditary spherocytosis and band 3 protein deficiency (band 3PRAGUE).The Journal of clinical investigation · 1994Article
- Deoxygenation affects fluorescence photobleaching recovery measurements of red cell membrane protein lateral mobility.Biophysical journal · 1994Article
- Band 3 and glycophorin are progressively aggregated in density-fractionated sickle and normal red blood cells. Evidence from rotational and lateral mobility studies.The Journal of clinical investigation · 1993Article
- Rotational dynamics of type I Fc epsilon receptors on individually-selected rat mast cells studied by polarized fluorescence depletion.Biophysical journal · 1992Article
- Nanosecond fluorescence microscopy. Emission kinetics of fura-2 in single cells.Biophysical journal · 1991Article
- Rotational diffusion of acetylcholine receptors on cultured rat myotubes.The Journal of cell biology · 1990Article
- Measurement of protein rotational motion using frequency domain polarized fluorescence depletion.Biophysical journal · 1988Article
- Polarized fluorescence photobleaching recovery for measuring rotational diffusion in solutions and membranes.Biophysical journal · 1988Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
A microscope-based system is described for directly measuring protein rotational motion in viscous environments such as cell membranes by polarized fluorescence depletion (PFD). Proteins labeled with fluorophores having a high quantum yield for triplet formation, such as eosin isothiocyanate (EITC), are examined anaerobically in a fluorescence microscope. An acousto-optic modulator generates a several-microsecond pulse of linearly polarized light which produces an orientationally-asymmetric depletion of ground state fluorescence in the sample. When the sample is then probed with light polarized parallel to the excitation pulse, fluorescence recovers over 0-1,000 microseconds as the sum of two exponentials. One exponential corresponds to triplet decay and the other to the rotational relaxation. An exciting pulse perpendicular to the probe beam is then applied. Fluorescence recovery following this pulse is the difference of the same two exponentials. Equations for fluorescence recovery kinetics to be expected in various experimentally significant cases are derived. Least-squares analysis using these equations then permits the triplet lifetime and rotational correlation time to be determined directly from PFD data. Instrumentation for PFD measurements is discussed that permits photobleaching recovery measurements of lateral diffusion coefficients using the same microscope system. With this apparatus, both rotational and translational diffusion coefficients (Dr, Dt) were measured for EITC-labeled bovine serum albumin in glycerol solutions. Values obtained for Dr and Dt are discussed in light of both the PFD models and the experimental system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.