Evidence map›Paper›PMID 37303717›Full record

ArticleFrontiers in veterinary science2023

The effects of ambient temperature exposure on feline fecal metabolome.

Olivia Chiu, Moran Tal, Abbinash Sanmugam, Myriam Hesta, Diego E Gomez, Jeffrey Scott Weese, Adronie Verbrugghe

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Olivia ChiuDepartment of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Moran TalDepartment of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Abbinash SanmugamDepartment of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Myriam HestaDepartment of Morphology, Imaging, Orthopedics, Rehabilitation and Nutrition, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Diego E GomezDepartment of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Jeffrey Scott WeeseDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Adronie VerbruggheDepartment of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
University of Guelph · CAGhent University · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: The fecal metabolome provides insight into overall gastrointestinal and microbial health. Methods for fecal sample storage in metabolomics research vary, however, making comparisons within current literature difficult. This study investigated the effect of ambient temperature exposure on microbial-derived metabolites of feline fecal samples. Methods: Fecal samples were collected from 11 healthy cats from a local boarding facility. Samples were manually homogenized and aliquoted. The first aliquot was frozen at -80°C within 1 hour of defecation, and remaining samples were exposed to ambient temperature for 2, 4, 6, 8, 12, and 24 h prior to freezing at -80°C. Fecal metabolites were quantified using Results: Concentrations of 20 out of 50 metabolites significantly differed due to ambient temperature exposure (7 amino acids, 6 fatty acids, 2 alcohols, 1 nitrogenous base, 4 miscellaneous). The earliest detected changes occurred 6 h post-defecation for cadaverine and fumaric acid. Discussion: This study shows ambient temperature exposure alters the composition of the feline fecal metabolome, but short-term (up to 4 h) exposure prior to storage in the freezer seems to be acceptable.

Indexed as

1 H nuclear magnetic resonance spectroscopyamino acidscatfecal metabolitesfecal storage conditionsvolatile fatty acids

Identifiers

PMID37303717
PMCPMC10250732
OpenAlexW4378471400

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.