Evidence map›Paper›PMID 37303052›Full record

ArticleBMC infectious diseases2023

Comparison of endotracheal aspirate and bronchoalveolar lavage fluid metagenomic next-generation sequencing in severe pneumonia: a nested, matched case-control study.

Renren Bao, Qing Mei, Tianjun Yang, Lei Zhang, Chunyan Zhu, Xiaoqin Fan, Yinzhong Wang, Fei Tong, Yuxi He, Xiaowei Fang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC infectious diseases, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Renren Bao *Department of Critical Care Medicine, The Affiliated Provincial Hospital of Anhui Medical University, Hefei, 230001, Anhui, China.
Qing Mei *Department of Critical Care Medicine, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, Anhui, China. qingmei@fsyy.ustc.edu.cn.
Tianjun Yang *Department of Critical Care Medicine, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, Anhui, China.
Lei ZhangDepartment of Critical Care Medicine, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, Anhui, China.
Chunyan ZhuDepartment of Critical Care Medicine, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, Anhui, China.
Xiaoqin FanDepartment of Critical Care Medicine, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, Anhui, China.
Yinzhong WangDepartment of Critical Care Medicine, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, Anhui, China.
Fei TongDepartment of Critical Care Medicine, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, Anhui, China.
Yuxi HeDepartment of Critical Care Medicine, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, Anhui, China.
Xiaowei FangDepartment of Critical Care Medicine, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, Anhui, China.
Shike GengDepartment of Critical Care Medicine, The Affiliated Provincial Hospital of Anhui Medical University, Hefei, 230001, Anhui, China.
Yu YangDepartment of Critical Care Medicine, The Affiliated Provincial Hospital of Anhui Medical University, Hefei, 230001, Anhui, China.
Ximei ShengWanNan Medical College, Wuhu, 241002, Anhui, China.
Aijun PanDepartment of Critical Care Medicine, The Affiliated Provincial Hospital of Anhui Medical University, Hefei, 230001, Anhui, China. aijunpan@fsyy.ustc.edu.cn.

Funding

the Fundamental Research Funds for the Central Universities WK9110000102the Key Research and Development Program of Anhui Province 202104j07020043
6 · The paper itself

Abstract

objectivesTo compare clinical outcomes in patients with severe pneumonia according to the diagnostic strategy used.

methodsIn this retrospective, nested, case-control study, patients with severe pneumonia who had undergone endotracheal aspirate (ETA) metagenomic next-generation sequencing of (mNGS) testing (n = 53) were matched at a ratio of 1 to 2 (n = 106) by sex, age, underlying diseases, immune status, disease severity scores, and type of pneumonia with patients who had undergone bronchoalveolar lavage fluid (BALF) mNGS. The microbiological characteristics and patient's prognosis of the two groups were compared.

resultsAn overall comparison between the two groups showed no significant differences in bacterial, fungal, viral, or mixed infections. However, subgroup analysis of 18 patients who received paired ETA and BALF mNGS showed a complete agreement rate for the two specimens of 33.3%. There were more cases for whom targeted treatment was initiated (36.79% vs. 22.64%; P = 0.043) and fewer cases who received no clinical benefit after mNGS (5.66% vs. 15.09%; P = 0.048) in the BALF group. The pneumonia improvement rate in the BALF group was significantly higher than in the ETA group (73.58% vs. 87.74%, P = 0.024). However, there were no significant differences in ICU mortality or 28-day mortality.

conclusionsWe do not recommend using ETA mNGS as the first-choice method for analyzing airway pathogenic specimens from severe pneumonia patients.

Indexed as

PneumoniaBronchoalveolar Lavage FluidCase-Control StudiesHigh-Throughput Nucleotide SequencingHumansRetrospective StudiesBronchoalveolar lavage fluidEndotracheal aspirateIntensive care unitNext-generation sequencingSevere pneumonia

Identifiers

PMID37303052
PMCPMC10258078

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.