Evidence map›Paper›PMID 37302818›Full record

ArticleCancer science2023

CD30 induces Reed-Sternberg cell-like morphology and chromosomal instability in classic Hodgkin lymphoma cell lines.

Mariko Watanabe, Hiromi Hatsuse, Kazuaki Nagao, Makoto Nakashima, Kaoru Uchimaru, Makoto Otsu, Koji Miyazaki, Ryouichi Horie

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. A CD4Nature communications · 2026
    Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Mariko WatanabeDivision of Hematology, Department of Laboratory Sciences, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.
Hiromi HatsuseDepartment of Molecular Genetics, School of Medicine, Kitasato University, Sagamihara, Japan.
Kazuaki NagaoDepartment of Molecular Genetics, School of Medicine, Kitasato University, Sagamihara, Japan.
Makoto NakashimaLaboratory of Tumor Cell Biology, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.
Kaoru UchimaruLaboratory of Tumor Cell Biology, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.
Makoto OtsuDivision of Hematology, Department of Laboratory Sciences, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.
Koji MiyazakiDepartment of Molecular Cell Therapy, Kitasato University Graduate School of Medical Sciences, Sagamihara, Japan.
Ryouichi HorieDivision of Hematology, Department of Laboratory Sciences, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.ORCID https://orcid.org/0000-0002-2431-2290
Kitasato University · JPThe University of Tokyo · JPKanagawa Institute of Technology · JP

Funding

Makoto Nakashima MEXT/JSPS KAKENHI (19K16580 and 22K15420)Mariko Watanabe MEXT/JSPS KAKENHI (19K07442)Ryouichi Horie MEXT/JSPS KAKENHI (17K08728,20K07379,23K06429)
6 · The paper itself

Abstract

Classic Hodgkin lymphoma (cHL) is characterized by multinucleated cells called Reed-Sternberg (RS) cells and genetic complexity. Although CD30 also characterizes cHL cells, its biological roles are not fully understood. In this report, we examined the link between CD30 and these characteristics of cHL cells. CD30 stimulation increased multinucleated cells resembling RS cells. We found chromatin bridges, a cause of mitotic errors, among the nuclei of multinucleated cells. CD30 stimulation induced DNA double-strand breaks (DSBs) and chromosomal imbalances. RNA sequencing showed significant changes in the gene expression by CD30 stimulation. We found that CD30 stimulation increased intracellular reactive oxygen species (ROS), which induced DSBs and multinucleated cells with chromatin bridges. The PI3K pathway was responsible for CD30-mediated generation of multinucleated cells by ROS. These results suggest that CD30 involves generation of RS cell-like multinucleated cells and chromosomal instability through induction of DSBs by ROS, which subsequently induces chromatin bridges and mitotic error. The results link CD30 not only to the morphological features of cHL cells, but also to the genetic complexity, both of which are characteristic of cHL cells.

Indexed as

Hodgkin DiseaseReed-Sternberg CellsCell LineChromatinChromosomal InstabilityHumansKi-1 AntigenPhosphatidylinositol 3-KinasesReactive Oxygen SpeciesChromatinKi-1 AntigenPhosphatidylinositol 3-KinasesReactive Oxygen SpeciesCD30chromosomal instabilityclassic Hodgkin lymphomareactive oxygen speciesReed-Sternberg cell

Identifiers

PMID37302818
PMCPMC10394143
OpenAlexW4380290639

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.