ArticleScientific reports2023
Comparison of pathogen detection consistency between metagenomic next-generation sequencing and blood culture in patients with suspected bloodstream infection.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 31 citations in OpenAlex.
- Diagnostic value of plasma cell-free DNA metagenomic next-generation sequencing in patients with suspected infections and exploration of clinical scenarios-a retrospective study from a single center.Annals of medicine · 2026Article
- Microbial signal profiles and organism-level concordance between plasma metagenomic sequencing and blood culture in suspected bloodstream infection.World journal of microbiology & biotechnology · 2026Article
- Occupational exposure to freshwater crustaceans as a route of paragonimiasis presenting with eosinophilic pleural effusion misdiagnosed as tuberculous pleurisy: A case report and literature review.Experimental and therapeutic medicine · 2026Article
- Influence of Ongoing Antibiotic Therapy on the Detection of Pathogenic Microorganisms Using Metagenomic Next-Generation Sequencing and Blood Culture in ICU Patients.Journal of clinical medicine · 2026Article
- Clinical efficacy of plasma cell-free DNA metagenomic next-generation sequencing in diagnosing bloodstream infections.BMC infectious diseases · 2026Article
- Comprehensive analysis of microorganisms in severe septic patients in the intensive care unit by mNGS and microbial culture.BMC microbiology · 2026Article
- Performance of metagenomic next-generation sequencing for bloodstream infections in perioperative critically ill patients- aFrontiers in cellular and infection microbiology · 2026Article
- Epidemiology, pathogen spectrum, and antimicrobial resistance of infections in burn patients stratified by total body surface area: a bicenter study with evaluation of targeted next-generation sequencing.Frontiers in microbiology · 2026Article
- Complementary Utility of Metagenomic Next-Generation Sequencing and Blood Culture for Bloodstream Infections: Insights into Pathogen-Specific Inflammatory and Coagulation Profiles.Infection and drug resistance · 2026Article
- Brucellosis Complicated by Thyroid Abscess and Life-Threatening Hemophagocytic Syndrome: A Case Report.Infection and drug resistance · 2026Article
- Next-Generation Sequencing for Bloodstream Infections: Shaping the Future of Rapid Diagnostics and Precision Medicine.Diagnostics (Basel, Switzerland) · 2025Review
- Metagenomic Next-Generation Sequencing in Infectious Diseases: Clinical Applications, Translational Challenges, and Future Directions.Diagnostics (Basel, Switzerland) · 2025Review
- Clinical utility of metagenomic next-generation sequencing in pathogen detection for lower respiratory tract infections.Scientific reports · 2025Article
- Assessment of Bacterial Community and Other Microorganism Along the Lam Takhong Watercourse, Nakhon Ratchasima, Thailand.Current microbiology · 2025Article
- The value of metagenomic next-generation sequencing in the diagnosis of fever of unknown origin.Scientific reports · 2025Article
- Application of Metagenomic Next-Generation Sequencing in HIV-Infected Patients with Bloodstream Infections.Infection and drug resistance · 2025Article
- Diagnosis and treatment ofIDCases · 2025Article
- Development and performance evaluation of a clinical metagenomics approach for identifying pathogens in the whole blood from patients with undifferentiated fever.Frontiers in cellular and infection microbiology · 2025Article
- Liver abscess and splenic infarction due to Yersinia pseudotuberculosis bloodstream infection: a case report.BMC infectious diseases · 2024Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of metagenomic next-generation sequencing (mNGS) has gradually been carried out by clinical practitioner. However, few studies have compared it with blood cultures in patients suffering from suspected bloodstream infections. The purpose of this study was to compare the detection of pathogenic microorganisms by these two assays in patients with suspected bloodstream infection. We retrospectively studied patients with fever, chills, antibiotic use for more than 3 days, suspected bloodstream infection, and admission to the emergency department of Ruijin Hospital from January 2020 to June 2022. All patients had blood drawn on the same day for blood mNGS and blood cultures. Clinical and laboratory parameters were collected on the day blood was drawn. The detection of pathogenic microorganisms by the two methods was compared. Risk factors and in-hospital mortality in patients with bloodstream infections were analysed separately for these two assays. In all 99 patients, the pathogenic microorganisms detection rate in blood mNGS was significantly higher than that in blood culture. Blood mNGS was consistent with blood culture in only 12.00% of all positive bacterial and fungal test results. The level of CRP is related to bacteraemia, fungaemia and viraemia detected by blood mNGS. No clear risk factors could be found in patients with a positive blood culture. In critically ill patients, both tests failed to improve patient outcomes. In patients with suspected bloodstream infection, mNGS is not yet a complete replacement for blood cultures.
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