ArticleMethods in molecular biology (Clifton, N.J.)2023
A Simple Ca
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Abstract
Rapid advances in light microscopy and development of all-optical electrophysiological imaging tools have greatly leveraged the speed and the depth of neurobiology studies. Calcium imaging is a common method that is useful for measuring calcium signals in cells and has been used as a functional proxy for neuronal activity. Here I describe a simple, stimulation-free approach that measures neuronal network activity and single-neuron dynamics in human neurons. This protocol provides the experimental workflow that includes step-wise illustrations of sample preparations, data processing, and analyses that can be used for quick phenotypical assessment and serves as a quick functional readout for mutagenesis or screen effort for neurodegenerative studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.