Evidence map›Paper›PMID 37300773›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

A Three-Dimensional Primary Cortical Culture System Compatible with Transgenic Disease Models, Virally Mediated Fluorescence, and Live Microscopy.

Sophie Brown, Elaina Atherton, David A Borton

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sophie BrownCenter for Biomedical Engineering, Brown University, Providence, RI, USA.
Elaina AthertonDepartment of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI, USA.
David A BortonCenter for Biomedical Engineering, Brown University, Providence, RI, USA. david_borton@brown.edu.

Funding

Bioengineering a cortical microtissue model to study human microglia in Alzheimer's diseaseR21AG077697 · NIA · BROWN UNIVERSITY · PI BORTON, DAVID ALLENSON, HUANG, YU-WEN ALVIN · 2022 to 2023
$413k
NIA NIH HHS R21 AG077697
6 · The paper itself

Abstract

In vitro cell culture models can offer high-resolution and high-throughput experimentation of cellular behaviors. However, in vitro culture approaches often fail to fully recapitulate complex cell processes involving synergistic interactions between heterogeneous neural cell populations and the surrounding neural microenvironment. Here, we describe the formation of a three-dimensional primary cortical cell culture system compatible with live confocal microscopy.

Indexed as

Cell Culture TechniquesNeuronsAnimalsAnimals, Genetically ModifiedImaging, Three-DimensionalMicroscopy, ConfocalMicroscopy, FluorescenceIn vitroLive imagingPrimary cell cultureThree-dimensional cell culture

Identifiers

PMID37300773

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.