Evidence map›Paper›PMID 37300767›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

The Generation and Functional Characterization of Human Microglia-Like Cells Derived from iPS and Embryonic Stem Cells.

Mikael Lehoux, Kevin Connolly, Benedetta Assetta, Yu-Wen Alvin Huang

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mikael LehouxDepartment of Molecular Biology, Cell Biology and Biochemistry, Center for Translational Neuroscience, Carney Institute for Brain Science and Brown Institute of Translational Science, Brown University, Providence, RI, USA.
Kevin ConnollyDepartment of Molecular Biology, Cell Biology and Biochemistry, Center for Translational Neuroscience, Carney Institute for Brain Science and Brown Institute of Translational Science, Brown University, Providence, RI, USA.
Benedetta AssettaDepartment of Molecular Biology, Cell Biology and Biochemistry, Center for Translational Neuroscience, Carney Institute for Brain Science and Brown Institute of Translational Science, Brown University, Providence, RI, USA.
Yu-Wen Alvin HuangDepartment of Molecular Biology, Cell Biology and Biochemistry, Center for Translational Neuroscience, Carney Institute for Brain Science and Brown Institute of Translational Science, Brown University, Providence, RI, USA. alvinhuang@brown.edu.

Funding

Stem Cells and AgingP20GM119943 · NIGMS · RHODE ISLAND HOSPITAL · PI QUESENBERRY, PETER J. · 2017 to 2021
$10.6M
Predoctoral Training in Molecular, Cellular, and Biochemical SciencesT32GM136566 · NIGMS · BROWN UNIVERSITY · PI Mark Aikens Johnson, Erica Nicole Larschan · 2020 to 2026
$3.1M
Bioengineering a cortical microtissue model to study human microglia in Alzheimer's diseaseR21AG077697 · NIA · BROWN UNIVERSITY · PI BORTON, DAVID ALLENSON, HUANG, YU-WEN ALVIN · 2022 to 2023
$413k
NIA NIH HHS R21 AG077697NIGMS NIH HHS P20 GM119943NIGMS NIH HHS T32 GM136566
6 · The paper itself

Abstract

The following protocol describes the generation of microglia cells from human-induced pluripotent stem cells (hiPSCs) using commercially available kits by StemCell Technologies. This protocol consists of three major steps: (1) Differentiation of hematopoietic precursor cells, (2) Microglia differentiation, and (3) Microglia maturation. Assays are described to characterize hematopoietic precursor cells and mature microglia.

Indexed as

Induced Pluripotent Stem CellsPluripotent Stem CellsCell DifferentiationEmbryonic Stem CellsHematopoietic Stem CellsHumansMicrogliaChemically defined differentiationHematopoietic precursor cellsInduced pluripotent stem (iPS) cellsMicroglia (iMG)

Identifiers

PMID37300767

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.