Evidence map›Paper›PMID 37300712›Full record

ArticleApplied microbiology and biotechnology2023

Tuning Thermostability and Catalytic Efficiency of Aflatoxin-Degrading Enzyme by Error-prone PCR.

Ru Jia, Senmiao Tian, Zhaofeng Yang, Faizan Ahmed Sadiq, Lan Wang, Simeng Lu, Guohua Zhang, Jianhui Li

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 10 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Ru JiaSchool of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan, 030006, China. sxdxjiaru@sxu.edu.cn.ORCID http://orcid.org/0000-0002-7108-0111
Senmiao TianSchool of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan, 030006, China.
Zhaofeng YangSchool of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan, 030006, China.
Faizan Ahmed SadiqFisheries and Food, Technology & Food Science Unit, Flanders Research Institute for Agriculture, 9090, Melle, Belgium.
Lan WangSchool of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan, 030006, China.
Simeng LuSchool of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan, 030006, China.
Guohua ZhangSchool of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan, 030006, China.
Jianhui LiCollege of Animal Sciences, Shanxi Agriculture University, Taigu, 030801, China.
Shanxi University · CNShanxi Agricultural University · CN

Funding

National Natural Science Foundation of China 31902198
6 · The paper itself

Abstract

In our previous work, a recombinant aflatoxin-degrading enzyme derived from Myxococcus fulvus (MADE) was reported. However, the low thermal stability of the enzyme had limitations for its use in industrial applications. In this study, we obtained an improved variant of recombinant MADE (rMADE) with enhanced thermostability and catalytic activity using error-prone PCR. Firstly, we constructed a mutant library containing over 5000 individual mutants. Three mutants with T

Indexed as

AflatoxinsCloning, MolecularEnzyme StabilityMutationPolymerase Chain ReactionTemperatureAflatoxinsAflatoxin degradation enzyme from Myxococcus fulvusCatalytic aflatoxin activityError-prone PCRProtein three-dimensional structural analysisThermostability

Identifiers

PMID37300712
OpenAlexW4380153806

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.