Fabrication of Multilayer Microfluidic Arrays for Passive, Efficient DNA Trapping and Profiling.

Christine M O'Keefe, Tza-Huei Jeff Wang

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 80% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Christine M O'KeefeJohns Hopkins University, Whiting School of Engineering, Department of Biomedical Engineering, Baltimore, MD, USA.

Tza-Huei Jeff WangJohns Hopkins University, Whiting School of Engineering, Department of Biomedical Engineering, Baltimore, MD, USA. thwang@jhu.edu.

Johns Hopkins Medicine · USJohns Hopkins University · US

Funding

A "Culture" Shift: Integrated Bacterial Screening and Antibacterial Susceptibility Test on Microfluidic Digital Array for Bloodstream InfectionsR01AI137272 · NIAID · JOHNS HOPKINS UNIVERSITY · PI WANG, TZA-HUEI JEFF, YANG, SAMUEL · 2018 to 2022

$3.6M

Ultrasensitive Detection of Tumor Specific DNA Methylation Changes for the Early Detection of Lung Cancer U01CA214165 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI HERMAN, JAMES G., WANG, TZA-HUEI JEFF · 2016 to 2021

$3.1M

Facile screening for esophageal cancer in LMICsUG3CA211457 · NCI · JOHNS HOPKINS UNIVERSITY · PI MELTZER, STEPHEN J, WANG, TZA-HUEI JEFF · 2017 to 2018

$861k

NCI NIH HHS U01 CA214165NCI NIH HHS UG3 CA211457NIAID NIH HHS R01 AI137272

6 · The paper itself

Abstract

Trace amounts of cell-free DNA containing cancer-specific biomarkers can be found in blood plasma. Detection of these biomarkers holds tremendous potential for applications such as noninvasive cancer diagnostics and therapeutic monitoring. However, such DNA molecules are extremely rare, and a typical patient blood sample may only contain a few copies. Here we describe the fabrication and operation of a microfluidic device to efficiently trap single DNA molecules into chambers for detection of tumor-specific biomarkers through a passive, geometric manipulation strategy.

Indexed as

Microfluidic Analytical TechniquesNeoplasmsBiomarkers, TumorDNAHumansLab-On-A-Chip DevicesMicrofluidicsBiomarkers, TumorDNACirculating DNADigital meltDigital PCRHeterogeneityMicrofluidic DNA capture efficiencyMolecule-by-molecule profilingPassive microfluidics

Identifiers

PMID37300626

DOI10.1039/B813709A

OpenAlexW4380152494

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.