Evidence map›Paper›PMID 37300611›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Functional Interrogation of Ca

Chenyu Liang, Miao Huang, Mai Tanaka, Suzanne Lightsey, Madison Temples, Sharon E Lepler, Peike Sheng, William P Mann, Adrienne E Widener, Dietmar W Siemann and 6 more

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.8field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. YAP at the Crossroads of Biomechanics and Drug Resistance in Human Cancer.International journal of molecular sciences · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 2 institutions in 2 countries.

Chenyu Liang *Department of Mechanical & Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, USA.
Miao Huang *Department of Mechanical & Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, USA.
Mai TanakaDepartment of Radiation Oncology, College of Medicine, University of Florida, Gainesville, FL, USA.
Suzanne LightseyDepartment of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, USA.
Madison TemplesDepartment of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, USA.
Sharon E LeplerDepartment of Radiation Oncology, College of Medicine, University of Florida, Gainesville, FL, USA.
Peike ShengDepartment of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA.
William P MannDepartment of Mechanical & Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, USA.
Adrienne E WidenerDepartment of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, USA.
Dietmar W SiemannDepartment of Radiation Oncology, College of Medicine, University of Florida, Gainesville, FL, USA.
Blanka SharmaDepartment of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, USA.
Mingyi XieDepartment of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA.
Yao DaiDepartment of Radiation Oncology, College of Medicine, University of Florida, Gainesville, FL, USA.
Edward PhelpsDepartment of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, USA.
Bo ZengInstitute of Cardiovascular Research, Southwest Medical University, Luzhou, China. zengbo@swmu.edu.cn.
Xin TangDepartment of Mechanical & Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, USA. xin.tang@ufl.edu.
University of Florida · USSouthwest Medical University · CN

Funding

RNA metabolism mediated by the Integrator complexR35GM128753 · NIGMS · UNIVERSITY OF FLORIDA · PI MINGYI XIE · 2018 to 2026
$4.0M
NIGMS NIH HHS R35 GM128753
6 · The paper itself

Abstract

Genetically encoded calcium indicators (GECIs) and high-resolution confocal microscopy enable dynamic visualization of calcium signals in cells and tissues. Two-dimensional and 3D biocompatible materials mimic the mechanical microenvironments of tumor and healthy tissues in a programmable manner. Cancer xenograft models and ex vivo functional imaging of tumor slices reveal physiologically relevant functions of calcium dynamics in tumors at different progression stages. Integration of these powerful techniques allows us to quantify, diagnose, model, and understand cancer pathobiology. Here, we describe detailed materials and methods used to establish this integrated interrogation platform, from generating transduced cancer cell lines that stably express CaViar (GCaMP5G + QuasAr2) to in vitro and ex vivo calcium imaging of the cells in 2D/3D hydrogels and tumor tissues. These tools open the possibility for detailed explorations of mechano-electro-chemical network dynamics in living systems.

Indexed as

CalciumNeoplasmsCalcium SignalingCell LineColoring AgentsHumansIndicators and ReagentsMicroscopy, FluorescenceTumor MicroenvironmentCalciumColoring AgentsIndicators and Reagents2D and 3D hydrogelsCancer xenograft modelCRISPR/Cas9 gene editingGenetically encoded calcium indicator (GECIs)In vitro and ex vivo calcium imagingMechanobiologyOptogeneticsRetrovirus and lentivirus transduction

Identifiers

PMID37300611
OpenAlexW4380152640

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.