cDNA Cloning of Feline PIWIL1 and Evaluation of Expression in the Testis of the Domestic Cat.

Leanne Stalker, Alanna G Backx, Allison K Tscherner, Stewart J Russell, Robert A Foster, Jonathan LaMarre

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Leanne StalkerDepartment of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

Alanna G BackxDepartment of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.ORCID 0000-0002-1877-0252

Allison K TschernerDepartment of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

Stewart J RussellDepartment of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

Robert A FosterDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W12, Canada.

Jonathan LaMarreDepartment of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.ORCID 0000-0002-3956-3041

Funding

Michelson Prize and Grants D1112-F13Natural Sciences and Engineering Research Council 04396

6 · The paper itself

Abstract

The PIWI clade of Argonaute proteins is essential for spermatogenesis in all species examined to date. This protein family binds specific classes of small non-coding RNAs known as PIWI-interacting RNAs (piRNAs) which together form piRNA-induced silencing complexes (piRISCs) that are recruited to specific RNA targets through sequence complementarity. These complexes facilitate gene silencing through endonuclease activity and guided recruitment of epigenetic silencing factors. PIWI proteins and piRNAs have been found to play multiple roles in the testis including the maintenance of genomic integrity through transposon silencing and facilitating the turnover of coding RNAs during spermatogenesis. In the present study, we report the first characterization of PIWIL1 in the male domestic cat, a mammalian system predicted to express four PIWI family members. Multiple transcript variants of

Indexed as

Piwi-Interacting RNATestisAnimalsArgonaute ProteinsCatsCloning, MolecularDNA, ComplementaryEndonucleasesMaleMammalsProtein IsoformsRNA, Small InterferingArgonaute ProteinsDNA, ComplementaryEndonucleasesPiwi-Interacting RNAProtein IsoformsRNA, Small Interferinggametemale reproductive modelsPIWI pathwaysmall non-coding RNAspermatogenenesistestistransposable element

Identifiers

PMID37298298

DOI10.3390/ijms24119346

PMCPMC10253428

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.