ReviewInternational journal of molecular sciences2023
DNA Polymerases for Whole Genome Amplification: Considerations and Future Directions.
Review in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Recombinant Thermostable DNA Polymerases: Current Approaches to Production, Molecular Engineering, and Applications in Biotechnology and Diagnostics.International journal of molecular sciences · 2026Review
- Harmonizing single-cell 3D genome data with STARK and scNucleome.Genome biology · 2026Article
- Assessing Amplification Quality and Bias in MDA Methods Through Comparative Analysis of Short-Read Sequencing.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Differential performance of strategies for single-cell whole-genome amplification.Cell reports methods · 2025Article
- Decoding microbiome responses to quarantine potato wart disease: first insights into suppression and biocontrol by full-length 16S rRNA gene profiling and functional prediction.Frontiers in plant science · 2025Article
- A multicenter study on accuracy and reproducibility of nanopore sequencing-based genotyping of bacterial pathogens.Journal of clinical microbiology · 2024Article
- The virome of bubaline (Bubalus bubalis) tonsils reveals an unreported bubaline polyomavirus.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Strategies and procedures to generate chimeric DNA polymerases for improved applications.Applied microbiology and biotechnology · 2024Review
- Detecting life by behavior, the overlooked sensitivity of behavioral assays.Scientific reports · 2024Article
- Enhancing efficiency of protein language models with minimal wet-lab data through few-shot learning.Nature communications · 2024Article
- Expression and functional study of DNA polymerases fromFrontiers in microbiology · 2024Article
- FLED: a full-length eccDNA detector for long-reads sequencing data.Briefings in bioinformatics · 2023Article
- Special Issue on Whole Genome Amplification.International journal of molecular sciences · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
In the same way that specialized DNA polymerases (DNAPs) replicate cellular and viral genomes, only a handful of dedicated proteins from various natural origins as well as engineered versions are appropriate for competent exponential amplification of whole genomes and metagenomes (WGA). Different applications have led to the development of diverse protocols, based on various DNAPs. Isothermal WGA is currently widely used due to the high performance of Φ29 DNA polymerase, but PCR-based methods are also available and can provide competent amplification of certain samples. Replication fidelity and processivity must be considered when selecting a suitable enzyme for WGA. However, other properties, such as thermostability, capacity to couple replication, and double helix unwinding, or the ability to maintain DNA replication opposite to damaged bases, are also very relevant for some applications. In this review, we provide an overview of the different properties of DNAPs widely used in WGA and discuss their limitations and future research directions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.