Evidence map›Paper›PMID 37294637›Full record

ArticleCell reports2023

Dnmt3bas coordinates transcriptional induction and alternative exon inclusion to promote catalytically active Dnmt3b expression.

Mohd Saleem Dar, Isaiah K Mensah, Ming He, Sarah McGovern, Ikjot Singh Sohal, Hannah Christian Whitlock, Nina Elise Bippus, Madison Ceminsky, Martin L Emerson, Hern J Tan and 2 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. TheBiology · 2026
    Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Mohd Saleem DarDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
Isaiah K MensahDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
Ming HeDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
Sarah McGovernDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
Ikjot Singh SohalPurdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA; Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Hannah Christian WhitlockDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
Nina Elise BippusDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
Madison CeminskyDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
Martin L EmersonDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
Hern J TanDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
Mark C HallDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USA; Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.
Humaira GowherDepartment of Biochemistry, Purdue University, West Lafayette, IN 47907, USA; Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA. Electronic address: hgowher@purdue.edu.
Purdue University West Lafayette · US

Funding

Transgenic Mouse Core Facility Shared Resource (TMCF-SR)P30CA023168 · NCI · PURDUE UNIVERSITY WEST LAFAYETTE · PI Andrea L Kasinski · 1985 to 2026
$43.4M
Regulation of Dnmt3 Activity at Enhancers of Cell Identity Genes During DifferentiationR01GM118654 · NIGMS · PURDUE UNIVERSITY · PI GOWHER, HUMAIRA · 2017 to 2021
$1.6M
NCI NIH HHS P30 CA023168NIGMS NIH HHS R01 GM118654
6 · The paper itself

Abstract

Embryonic expression of DNMT3B is critical for establishing de novo DNA methylation. This study uncovers the mechanism through which the promoter-associated long non-coding RNA (lncRNA) Dnmt3bas controls the induction and alternative splicing of Dnmt3b during embryonic stem cell (ESC) differentiation. Dnmt3bas recruits the PRC2 (polycomb repressive complex 2) at cis-regulatory elements of the Dnmt3b gene expressed at a basal level. Correspondingly, Dnmt3bas knockdown enhances Dnmt3b transcriptional induction, whereas overexpression of Dnmt3bas dampens it. Dnmt3b induction coincides with exon inclusion, switching the predominant isoform from the inactive Dnmt3b6 to the active Dnmt3b1. Intriguingly, overexpressing Dnmt3bas further enhances the Dnmt3b1:Dnmt3b6 ratio, attributed to its interaction with hnRNPL (heterogeneous nuclear ribonucleoprotein L), a splicing factor that promotes exon inclusion. Our data suggest that Dnmt3bas coordinates alternative splicing and transcriptional induction of Dnmt3b by facilitating the hnRNPL and RNA polymerase II (RNA Pol II) interaction at the Dnmt3b promoter. This dual mechanism precisely regulates the expression of catalytically active DNMT3B, ensuring fidelity and specificity of de novo DNA methylation.

Indexed as

DNA (Cytosine-5-)-MethyltransferasesDNA MethylationAnimalsCell DifferentiationDNA Methyltransferase 3BEmbryonic Stem CellsExonsMicePolycomb Repressive Complex 2DNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3BPolycomb Repressive Complex 2alternative splicingchromatin modificationsCP: Molecular biologyDNA methylationdnmt3bDnmt3basenhancershnRNPLlncRNAPRC2 complexpromoters

Identifiers

PMID37294637
PMCPMC10592478
OpenAlexW4379877931

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.