Evidence map›Paper›PMID 37293698›Full record

ArticleDisease models & mechanisms2023

Analysis of the distal urinary tract in larval and adult zebrafish reveals homology to the human system.

Ibrahim Jubber, Duncan R Morhardt, Jonathan Griffin, Marcus G Cumberbatch, Maggie Glover, Yang Zheng, Ishtiaq Rehman, Catherine A Loynes, Syed A Hussain, Stephen A Renshaw and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Disease models & mechanisms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Modeling of Charcot-Marie-Tooth disease in zebrafish.Frontiers in molecular neuroscience · 2025
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 2 countries.

Ibrahim JubberDepartment of Oncology and Human Metabolism, University of Sheffield, Sheffield S10 2RX, UK.ORCID 0000-0002-4558-2521
Duncan R MorhardtDepartment of Molecular and Systems Biology, Dartmouth Cancer Center, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA.
Jonathan GriffinDepartment of Oncology and Human Metabolism, University of Sheffield, Sheffield S10 2RX, UK.
Marcus G CumberbatchDepartment of Oncology and Human Metabolism, University of Sheffield, Sheffield S10 2RX, UK.
Maggie GloverThe Bateson Centre and Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield S10 2TN, UK.
Yang ZhengDepartment of Molecular and Systems Biology, Dartmouth Cancer Center, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA.
Ishtiaq RehmanDepartment of Oncology and Human Metabolism, University of Sheffield, Sheffield S10 2RX, UK.
Catherine A LoynesThe Bateson Centre and Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield S10 2TN, UK.
Syed A HussainDepartment of Oncology and Human Metabolism, University of Sheffield, Sheffield S10 2RX, UK.
Stephen A RenshawThe Bateson Centre and Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield, Sheffield S10 2TN, UK.ORCID 0000-0003-1790-1641
Steven D LeachDepartment of Molecular and Systems Biology, Dartmouth Cancer Center, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA.
Vincent T CunliffeSchool of Biosciences, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK.ORCID 0000-0001-7483-7610
James W F CattoDepartment of Oncology and Human Metabolism, University of Sheffield, Sheffield S10 2RX, UK.ORCID 0000-0003-2787-8828
University of Sheffield · GBSheffield Teaching Hospitals NHS Foundation Trust · GBDartmouth College · USWeston Park Cancer Centre · GB

Funding

Department of Health
6 · The paper itself

Abstract

Little is known about the distal excretory component of the urinary tract in Danio rerio (zebrafish). This component is affected by many human diseases and disorders of development. Here, we have undertaken multi-level analyses to determine the structure and composition of the distal urinary tract in the zebrafish. In silico searches identified uroplakin 1a (ukp1a), uroplakin 2 (upk2) and uroplakin 3b (upk3b) genes in the zebrafish genome (orthologues to genes that encode urothelium-specific proteins in humans). In situ hybridization demonstrated ukp1a expression in the zebrafish pronephros and cloaca from 96 h post-fertilization. Haematoxylin and Eosin staining of adult zebrafish demonstrated two mesonephric ducts uniting into a urinary bladder that leads to a distinct urethral opening. Immunohistochemistry identified Uroplakin 1a, Uroplakin 2 and GATA3 expression in zebrafish urinary bladder cell layers that match human urothelial expression. Fluorescent dye injections demonstrated zebrafish urinary bladder function, including urine storage and intermittent micturition, and a urethral orifice separate from the larger anal canal and rectum. Our findings reveal homology between the urinary tracts of zebrafish and humans, and offer the former as a model system to study disease.

Indexed as

Membrane GlycoproteinsZebrafishAdultAnimalsHumansUrinary BladderUroplakin IaUroplakin IIMembrane GlycoproteinsUroplakin IaUroplakin IITetraspaninsUrethraUrinary bladderUrinary tractUroplakinsZebrafish

Identifiers

PMID37293698
PMCPMC10387350
OpenAlexW4379967315

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.