ArticleEnvironmental science & technology letters2020
Zika Virus RNA Persistence in Sewage.
Article in Environmental science & technology letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 58 citations in OpenAlex.
- Toward a quality-managed operational architecture for wastewater surveillance of zoonotic and emerging pathogens.Applied and environmental microbiology · 2026Review
- Alignment-freeJournal of clinical microbiology · 2026Article
- Environmental pathogen surveillance in cities without universal piped wastewater infrastructure.PLOS global public health · 2026Article
- Detection of Monkeypox Virus Clade Ib DNA in Wastewater Solids at Wastewater Treatment Plants, United States.Emerging infectious diseases · 2025Article
- Uncovering DENV, CHIKV, and ZIKV in Urban Wastewater in Brazil Through Genomic and Molecular Screening.Microorganisms · 2025Article
- The Protective Effect of Virus Capsids on RNA and DNA Virus Genomes in Wastewater.Environmental science & technology · 2023Article
- Persistence of Coronavirus on Surface Materials and Its Control Measures Using Nonthermal Plasma and Other Agents.International journal of molecular sciences · 2023Review
- Zika virus RNA persistence and recovery in water and wastewater: An approach for Zika virus surveillance in resource-constrained settings.Water research · 2023Article
- Article
- Persistence of Toscana virus in sugar and blood meals of phlebotomine sand flies: epidemiological and experimental consequences.Scientific reports · 2023Article
- Sources, fates and treatment strategies of typical viruses in urban sewage collection/treatment systems: A review.Desalination · 2022Article
- Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance.The Science of the total environment · 2022Review
- Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature.Pathogens (Basel, Switzerland) · 2021Article
- Decay of SARS-CoV-2 and surrogate murine hepatitis virus RNA in untreated wastewater to inform application in wastewater-based epidemiology.Environmental research · 2020Article
- Sewage analysis as a tool for the COVID-19 pandemic response and management: the urgent need for optimised protocols for SARS-CoV-2 detection and quantification.Journal of environmental chemical engineering · 2020Article
- Surveillance of SARS-CoV-2 RNA in wastewater: Methods optimisation and quality control are crucial for generating reliable public health information.Current opinion in environmental science & health · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Despite substantial advances in knowledge and understanding about Zika virus (ZIKV), limitations in surveillance for this mainly asymptomatic infection constrain attempts to characterize the epidemiological distribution of the virus. Monitoring of fecal waste streams including sewage offers opportunities to track the spread of arboviruses such as ZIKV, known to be present in fecal waste and urine. To demonstrate the feasibility of ZIKV RNA detection in sewage, we examined viral RNA decay in sewage from a local wastewater treatment plant. We added ZIKV (MEX 1-44) to unpasteurized sewage and stored the samples at 4°C, 25°C or 35°C for one month. We extracted nucleic acids from the mixture using a QiaAmp Minelute Virus Spin Kit and measured ZIKV RNA using a TaqPath Zika Virus Kit. We found no appreciable decline in ZIKV RNA detection at 4°C during the month. We estimate that 90% decay of detectable ZIKV RNA occurred after 21 days at 25°C and after 8.5 days at 35°C. Our preliminary work suggests ZIKV RNA can remain detectable in sewage over a range of temperatures and that sewage provides a cost-effective, community diagnostic tool that deserves further investigation as a novel epidemiologic surveillance approach.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.