Evidence map›Paper›PMID 37293075›Full record

ArticlebioRxiv : the preprint server for biology2023

An inducible tricolor reporter mouse for simultaneous imaging of lysosomes, mitochondria and microtubules.

Vera Hutchison, Anne Lynch, Andrés Mauricio Gutierrez Gamez, Jichao Chen

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Vera HutchisonDepartment of Pulmonary Medicine, the University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.
Anne LynchDepartment of Pulmonary Medicine, the University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.
Andrés Mauricio Gutierrez GamezTecnológico de Monterrey - Escuela de Medicina, Monterrey 64710, Mexico.
Jichao ChenDepartment of Pulmonary Medicine, the University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.
The University of Texas MD Anderson Cancer Center · USTecnológico de Monterrey · MX

Funding

Role of AT1 cells in perinatal lung maturationR01HL130129 · NHLBI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI CHEN, JICHAO · 2016 to 2023
$4.1M
Transcriptional and epigenetic basis of lung epithelial cell fateR01HL153511 · NHLBI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI CHEN, JICHAO · 2020 to 2023
$2.5M
Upgrading an existing NIKON A1-confocal with LUNV Laser Launch and DU-G detectorS10OD024976 · OD · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI LOZANO, GUILLERMINA · 2018 to 2018
$202k
NHLBI NIH HHS R01 HL130129NHLBI NIH HHS R01 HL153511NIH HHS S10 OD024976
6 · The paper itself

Abstract

Cell-type-specific use of the same DNA blueprint generates diverse cell types. Such diversity must also be executed via differential deployment of the same subcellular machinery. However, our understanding of the size, distribution, and dynamics of subcellular machinery in native tissues, and their connection to cellular diversity, remain limited. We generate and characterize an inducible tricolor reporter mouse, dubbed "kaleidoscope", for simultaneous imaging of lysosomes, mitochondria and microtubules in any cell type and at a single cell resolution. The expected subcellular compartments are labeled in culture and in tissues with no impact on cellular and organismal viability. Quantitative and live imaging of the tricolor reporter captures cell-type-specific organelle features and kinetics in the lung, as well as their changes after Sendai virus infection.

Indexed as

lamellar bodylysosomemicrotubulemitochondrionROSA reporter mousesubcellular structure

Identifiers

PMID37293075
PMCPMC10245888
OpenAlexW4377824071

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.