Evidence map›Paper›PMID 37293049›Full record

ArticlebioRxiv : the preprint server for biology2023

Chondroitin sulfate, dermatan sulfate, and hyaluronic acid differentially modify the biophysical properties of collagen-based hydrogels.

Marcos Cortes-Medina, Andrew R Bushman, Peter E Beshay, Jonathan J Adorno, Miles M Menyhert, Riley M Hildebrand, Shashwat S Agarwal, Alex Avendano, Jonathan W Song

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Marcos Cortes-MedinaDepartment of Biomedical Engineering, The Ohio State University, Columbus OH 43210.
Andrew R BushmanDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus OH 43210.
Peter E BeshayDepartment of Mechanical and Aerospace Engineering, The Ohio State University, Columbus OH 43210.
Jonathan J AdornoDepartment of Biomedical Engineering, The Ohio State University, Columbus OH 43210.
Miles M MenyhertDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus OH 43210.
Riley M HildebrandDepartment of Biomedical Engineering, The Ohio State University, Columbus OH 43210.
Shashwat S AgarwalDepartment of Mechanical and Aerospace Engineering, The Ohio State University, Columbus OH 43210.
Alex AvendanoDepartment of Biomedical Engineering, The Ohio State University, Columbus OH 43210.
Jonathan W SongDepartment of Mechanical and Aerospace Engineering, The Ohio State University, Columbus OH 43210.
The Ohio State University · US

Funding

Biophysical-based approach for controlling blood vessel structure and functionR01HL141941 · NHLBI · OHIO STATE UNIVERSITY · PI CASTRO, CARLOS E., PRAKASH, SHAURYA · 2018 to 2021
$2.0M
NHLBI NIH HHS R01 HL141941
6 · The paper itself

Abstract

Fibrillar collagens and glycosaminoglycans (GAGs) are structural biomolecules that are natively abundant to the extracellular matrix (ECM). Prior studies have quantified the effects of GAGs on the bulk mechanical properties of the ECM. However, there remains a lack of experimental studies on how GAGs alter other biophysical properties of the ECM, including ones that operate at the length scales of individual cells such as mass transport efficiency and matrix microstructure. Here we characterized and decoupled the effects of the GAG molecules chondroitin sulfate (CS) dermatan sulfate (DS) and hyaluronic acid (HA) on the stiffness (indentation modulus), transport (hydraulic permeability), and matrix microarchitecture (pore size and fiber radius) properties of collagen-based hydrogels. We complement these biophysical measurements of collagen hydrogels with turbidity assays to profile collagen aggregate formation. Here we show that CS, DS, and HA differentially regulate the biophysical properties of hydrogels due to their alterations to the kinetics of collagen self-assembly. In addition to providing information on how GAGs play significant roles in defining key physical properties of the ECM, this work shows new ways in which stiffness measurements, microscopy, microfluidics, and turbidity kinetics can be used complementary to reveal details of collagen self-assembly and structure.

Identifiers

PMID37293049
PMCPMC10245839
OpenAlexW4377693138

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.