Evidence map›Paper›PMID 37293017›Full record

ArticlebioRxiv : the preprint server for biology2023

Rapid, efficient auxin-inducible protein degradation in

Kedric L Milholland, Justin B Gregor, Smriti Hoda, Soledad Píriz-Antúnez, Encarnación Dueñas-Santero, Bao Gia Vu, Krishna P Patel, W Scott Moye-Rowley, Carlos R Vázquez de Aldana, Jaime Correa-Bordes and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Kedric L MilhollandDepartment of Biochemistry and Institute for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.
Justin B GregorDepartment of Biochemistry and Institute for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.
Smriti HodaDepartment of Biochemistry and Institute for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.
Soledad Píriz-AntúnezDepartment of Biomedical Sciences, Universidad de Extremadura, Badajoz, Spain.
Encarnación Dueñas-SanteroInstitute of Functional Biology and Genomics, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Salamanca (USAL), Salamanca, Spain.
Bao Gia VuDepartment of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA, 52242, USA.
Krishna P PatelDepartment of Biochemistry and Institute for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.
W Scott Moye-RowleyDepartment of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA, 52242, USA.
Carlos R Vázquez de AldanaInstitute of Functional Biology and Genomics, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Salamanca (USAL), Salamanca, Spain.
Jaime Correa-BordesDepartment of Biomedical Sciences, Universidad de Extremadura, Badajoz, Spain.
Scott D BriggsDepartment of Biochemistry and Institute for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.
Mark C HallDepartment of Biochemistry and Institute for Cancer Research, Purdue University, West Lafayette, IN 47907, USA.
Purdue University West Lafayette · USUniversity of Iowa · USConsejo Superior de Investigaciones Científicas · ESUniversidad de Extremadura · ES

Funding

Quality Assurance and Quality Control Project Management: Improving Submissions and Study Conduct in the Human Subjects Research Prior Approval ProcessUL1TR002529 · NCATS · INDIANA UNIVERSITY INDIANAPOLIS · PI MOE, SHARON M, WIEHE, SARAH ELIZABETH · 2018 to 2022
$27.2M
Cdc14 phosphatase - novel roles in drug resistance, virulence, and the response to cell wall stress in fungal pathogensR01AI168050 · NIAID · PURDUE UNIVERSITY · PI Mark C Hall · 2023 to 2026
$2.4M
Genetic analysis of pleiotropic drug resistanceR01AI152494 · NIAID · UNIVERSITY OF IOWA · PI MOYE-ROWLEY, W SCOTT · 2020 to 2024
$2.1M
SET Domain Epigenetic Factors Govern Antifungal Drug Efficacy and Fungal PathogenesisR01AI136995 · NIAID · PURDUE UNIVERSITY · PI BRIGGS, SCOTT D · 2018 to 2022
$1.9M
Drug Discovery in Infectious Disease TrainingT32AI148103 · NIAID · PURDUE UNIVERSITY · PI STAHELIN, ROBERT VIRGIL, YEO, YOON · 2020 to 2024
$949k
NCATS NIH HHS UL1 TR002529NIAID NIH HHS R01 AI136995NIAID NIH HHS R01 AI152494NIAID NIH HHS R01 AI168050NIAID NIH HHS T32 AI148103
6 · The paper itself

Abstract

A variety of inducible protein degradation (IPD) systems have been developed as powerful tools for protein functional characterization. IPD systems provide a convenient mechanism for rapid inactivation of almost any target protein of interest. Auxin-inducible degradation (AID) is one of the most common IPD systems and has been established in diverse eukaryotic research model organisms. Thus far, IPD tools have not been developed for use in pathogenic fungal species. Here, we demonstrate that the original AID and the second generation AID2 systems work efficiently and rapidly in the human pathogenic yeasts

Identifiers

PMID37293017
PMCPMC10245712
OpenAlexW4377098403

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.