Evidence map›Paper›PMID 37292957›Full record

ArticlebioRxiv : the preprint server for biology2024

Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release.

Xuehan Zhou, Carrie Stine, Patricia Oliveira Prada, Debora Fusca, Kevin Assoumou, Jan Dernic, Musadiq A Bhat, Ananya S Achanta, Joseph C Johnson, Amanda Loren Pasqualini and 13 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 7 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

23 authors at 5 institutions in 5 countries.

Xuehan ZhouInstitute of Pharmacology and Toxicology, University of Zürich, Zürich, CH.
Carrie StineCenter for the Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA, USA.
Patricia Oliveira PradaMax Planck Institute for Metabolism Research, Cologne, DE.
Debora FuscaExcellence Cluster on Cellular Stress Responses in Aging Associated Diseases (CECAD) and Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, DE.
Kevin AssoumouDepartment of Cell Physiology and Metabolism, University of Geneva, CH.
Jan DernicInstitute of Pharmacology and Toxicology, University of Zürich, Zürich, CH.
Musadiq A BhatInstitute of Pharmacology and Toxicology, University of Zürich, Zürich, CH.
Ananya S AchantaCenter for the Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA, USA.
Joseph C JohnsonCenter for the Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA, USA.
Amanda Loren PasqualiniCenter for the Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA, USA.
Sanjana JadhavCenter for the Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA, USA.
Corinna A BauderMax Planck Institute for Metabolism Research, Cologne, DE.
Lukas SteuernagelMax Planck Institute for Metabolism Research, Cologne, DE.
Luca RavottoInstitute of Pharmacology and Toxicology, University of Zürich, Zürich, CH.
Dietmar BenkeInstitute of Pharmacology and Toxicology, University of Zürich, Zürich, CH.
Bruno WeberInstitute of Pharmacology and Toxicology, University of Zürich, Zürich, CH.
Azra SukoCenter for the Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA, USA.
Richard D PalmiterCenter for the Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA, USA.
Miriam StoeberDepartment of Cell Physiology and Metabolism, University of Geneva, CH.
Peter KloppenburgExcellence Cluster on Cellular Stress Responses in Aging Associated Diseases (CECAD) and Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, DE.ORCID 0000-0002-4554-404X
Jens C BrüningMax Planck Institute for Metabolism Research, Cologne, DE.
Michael R BruchasCenter for the Neurobiology of Addiction, Pain, and Emotion, University of Washington, Seattle, WA, USA.ORCID 0000-0003-4713-7816
Tommaso PatriarchiInstitute of Pharmacology and Toxicology, University of Zürich, Zürich, CH.ORCID 0000-0001-9351-3734
University of Washington · USUniversity of Zurich · CHUniversity of Cologne · DEUniversity of Geneva · CHHoward Hughes Medical Institute · US

Funding

Project 4_Bruchas : Circuit-level Approaches for Dissecting Approach/Avoidance Behaviors Mediated by Nociceptin Systems in MiceP50MH119467 · NIMH · MCLEAN HOSPITAL · PI BRUCHAS, MICHAEL R. · 2020 to 2024
$15.9M
TRAINING IN THE MOLECULAR PHARMACOLOGY OF ABUSED DRUGST32DA007278 · NIDA · UNIVERSITY OF WASHINGTON · PI Susan Marie Ferguson, Paul E. M. Phillips · 1993 to 2026
$9.8M
Dissecting stress modulation of pnVTA nociceptin peptide-mediated approach-avoidance behaviorF31DA059438 · NIDA · UNIVERSITY OF WASHINGTON · PI STINE, CAROLYN ANN · 2023 to 2024
$90k
NIDA NIH HHS F31 DA059438NIDA NIH HHS T32 DA007278NIMH NIH HHS P50 MH119467
6 · The paper itself

Abstract

Nociceptin/orphanin-FQ (N/OFQ) is a recently appreciated critical opioid peptide with key regulatory functions in several central behavioral processes including motivation, stress, feeding, and sleep. The functional relevance of N/OFQ action in the mammalian brain remains unclear due to a lack of high-resolution approaches to detect this neuropeptide with appropriate spatial and temporal resolution. Here we develop and characterize NOPLight, a genetically encoded sensor that sensitively reports changes in endogenous N/OFQ release. We characterized the affinity, pharmacological profile, spectral properties, kinetics, ligand selectivity, and potential interaction with intracellular signal transducers of NOPLight

Identifiers

PMID37292957
PMCPMC10245933
OpenAlexW4378363680

What OpenQuestion holds

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LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.