Evidence map›Paper›PMID 37292907›Full record

ArticlebioRxiv : the preprint server for biology2023

CRISPR-based diagnostics detects invasive insect pests.

Pathour R Shashank, Brandon M Parker, Santosh R Rananaware, David Plotkin, Christian Couch, Lilia G Yang, Long T Nguyen, N R Prasannakumar, W Evan Braswell, Piyush K Jain and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Pathour R ShashankMcGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA.
Brandon M ParkerMcGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA.
Santosh R RananawareDepartment of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA.
David PlotkinMcGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA.
Christian CouchMcGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA.
Lilia G YangDepartment of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA.
Long T NguyenDepartment of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA.
N R PrasannakumarDivision of Crop Protection, ICAR-Indian Institute of Horticultural Research, Bengaluru 560089, India.
W Evan BraswellInsect Management and Molecular Diagnostics Laboratory, USDA APHIS PPQ S&T, 22675 North Moorefield Road, Edinburg, Texas 78541, USA.
Piyush K JainDepartment of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA.
Akito Y KawaharaMcGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA.

Funding

Discovery and engineering of CRISPR/Cas systemsR35GM147788 · NIGMS · UNIVERSITY OF FLORIDA · PI Piyush K Jain · 2022 to 2026
$1.8M
NIGMS NIH HHS R35 GM147788
6 · The paper itself

Abstract

Rapid identification of organisms is essential across many biological and medical disciplines, from understanding basic ecosystem processes and how organisms respond to environmental change, to disease diagnosis and detection of invasive pests. CRISPR-based diagnostics offers a novel and rapid alternative to other identification methods and can revolutionize our ability to detect organisms with high accuracy. Here we describe a CRISPR-based diagnostic developed with the universal cytochrome-oxidase 1 gene (CO1). The CO1 gene is the most sequenced gene among Animalia, and therefore our approach can be adopted to detect nearly any animal. We tested the approach on three difficult-to-identify moth species (

Indexed as

Cas12aCRISPRdiagnosticsgenetic identificationKeiferia lycopersicellaleaf minerPhthorimaea absolutaRPAScrobipalpa atriplicellaTuta absoluta

Identifiers

PMID37292907
PMCPMC10245733

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.