Evidence map›Paper›PMID 37292678›Full record

ArticleResearch square2023

Novel requirements for HAP2-mediated gamete fusion in

J F Pinello, J Loidl, E S Seltzer, D Cassidy-Hanley, D Kolbin, A Abdelatif, F A Rey, R An, N J Newberger, Y Bisharyan and 5 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 3 countries.

J F PinelloDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742 USA.
J LoidlDepartment of Chromosome Biology, Max Perutz Labs, University of Vienna, Vienna, Austria.
E S SeltzerDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, 14850 USA.
D Cassidy-HanleyDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, 14850 USA.
D KolbinDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, 14850 USA.
A AbdelatifDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, 14850 USA.
F A ReyUnité de Virologie Structurale, Institut Pasteur, 75724 Paris, France.ORCID 0000-0002-9953-7988
R AnDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, 14850 USA.
N J NewbergerDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, 14850 USA.
Y BisharyanOffice of Technology Development, Harvard University, Cambridge, MA, 02138 USA.
H PapoyanDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, 14850 USA.
H M ByunDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, 14850 USA.
H C AguilarDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, 14850 USA.ORCID 0000-0001-6879-8360
E ColeBiology Department, St. Olaf College, Northfield, MN, 55057 USA.
T G ClarkDepartment of Microbiology and Immunology, Cornell University, Ithaca, NY, 14850 USA.ORCID 0000-0002-7816-9899
Cornell University · USUniversity of Vienna · ATCentre National de la Recherche Scientifique · FRInstitut Pasteur · FR

Funding

A Resource Center for Tetrahymena ThermophilaP40OD010964 · OD · WASHINGTON UNIVERSITY · PI DOUGLAS LEE CHALKER · 2012 to 2026
$6.5M
Membrane proteins driving a cell-cell fusion reaction during fertilizationK99GM146112 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI PINELLO, JENNIFER FRICKE · 2022 to 2023
$225k
NIGMS NIH HHS K99 GM146112NIH HHS P40 OD010964
6 · The paper itself

Abstract

The ancestral gamete fusion protein, HAP2, catalyzes sperm-egg fusion in a broad range of taxa dating to the last eukaryotic common ancestor. Remarkably, HAP2 orthologs are structurally related to the class II fusogens of modern-day viruses, and recent studies make clear that these proteins utilize similar mechanisms to achieve membrane merger. To identify factors that may regulate HAP2 activity, we screened mutants of the ciliate

Indexed as

HAP2membrane fusionmembrane protrusionTetrahymenaZFR1

Identifiers

PMID37292678
PMCPMC10246275
OpenAlexW4376618315

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.